Lecture 9 Antibacterial drugs affecting nucleic acids/nucleic acid biosynthesis Flashcards
Give an example of drugs that inhibit tetrahydrofolate biosynthesis
sulphonamides
trimethoprim
Give an example of drugs that inhibit bacterial DNA supercoiling and decatenation
Quinolones inc. fluoroquinolones
Give an example of drugs that cause bacterial DNA damage
nitroimidazoles and nitrofurans e.g. methronidazole, nitrofurantoin
Give an example of drugs that inhibit RNA synthesis
rifamycins
Why is tetrahydrofolate production important in bacteria
essential for the production of DNA
Which step in the pathway of tetrahydrofolate production does sulfonamides inhibit?
GTP to dihydropteroate
Inhibits dihydropteroate synthase by binding to the PABA binding site (analogue) at a higher affinity
Which step in the pathway of tetrahydrofolate production does trimethoprim inhibit?
Dihydrofolate to tetrahydrofolate
Inhibits dihydrofolate reductase enzyme (analogue)
What is the advantage of using trimethoprim and sulfonamides together?
used alone = bacteriostatic
Used together = bacteriocidal (synergistic effect)
How are sulfonamides selective to bacteria
Humans do also need tetrahydrofolate but the pathway of production is different
GTP to dihydropteroate does not exist in the human pathway
What are the clinical uses of trimethoprim and sulfonamides?
First line treatment/prophylaxis for pneumocystis jiroveci pneumonia in HIV
UTI
Sometimes resp and GI tract infections and malaria due to plasmodium falciparum
What are side effects associated with sulfonamides
Hypersensitivity
Drug induced fever
Steven-Johnson syndrome
Haemolytic anaemia in patients with inherited glucose-6-phosphate deficiency in red blood cells
What are side effects associated with trimethorpim
rash
nausea
vomiting
hypersensitivity
What is the mode of action of quinolones and fluroquinolones?
Target DNA gyrase (gyrA, gyrB) and topisomerase IV (parC, parE)
Specifically binds into the ‘quinolone binding pocket’ - where the staggered cuts have been made via base stacking = complex can no longer rejoin
Therefore supercoiling and decatenation does not occur
Blocks DNA replication/transcription therefore Bacteriacidal
What is the function of DNA gyrase?
Catalyses ATP dependent DNA double strand breakage/rejoining reactions
Cuts at 4 base pair staggered sites on the double stranded DNA - the enzyme then binds to the 5’ end via a tyrosine residue
-ve supercoiling - relax DNA is coiled so that it can be packaged in bacteria
What is the function of topoisomerase IV?
Catalyses ATP dependent DNA double strand breakage/rejoining reactions
Cuts at 4 base pair staggered sites on the double stranded DNA - the enzyme then binds to the 5’ end via a tyrosine residue
Decatination - After replication of chromosome, the 2 daughter chromosomes interlink