Lecture 8

Question 1

Endoplasmic Reticulum (ER)

Answer 1

Network of membrane-bound compartments isn’t eh cytoplasm of eukaryotic cells
- location of majority of liquids and membrane and secretory proteins are synthesized

Question 2

Parts of the ER

Answer 2

ER membrane and Lumen

Question 3

ER membrane

Answer 3

Comprises 50-90% of a cell’s membrane

- separates the ER from the cytosol of the cell

Question 4

ER Lumen

Answer 4

Area enclosed by the endoplasmic reticulum’s membrane

- makes up about 15% of a eukaryotic cell’s total volume

Question 5

Ribosomes

Answer 5

Protein synthesizing units in a cell

Question 6

Isolation and Purification of ER

Answer 6

• homogenize/lyse/split the cell
• complexity and size of the ER causes it to break into small fragments = called microsomes
  Smooth microsomes =low density
  Rough Microsomes = high density