Lecture 7: Protein Function Flashcards
1
Q
Rate of association
A
ka
2
Q
Rate of dissociation
A
kd
3
Q
Association constant (Ka)
A
Measurement of the affinity of ligand for a protein
4
Q
Higher numerical value of Ka=
A
Higher affinity binding of the ligand to protein
5
Q
Ka is the ratio of
A
The association (ka) and dissociation (kd)
6
Q
First order reaction
A
(PL)= P + L
7
Q
Second order reaction
A
P + L = PL
8
Q
Kd=
A
1/Ka
9
Q
The more tightly a protein binds its ligand…
A
The lower the numerical value
10
Q
Dissociation constant
A
KD= (P)(L)/(PL)
11
Q
Fraction protein bound
A
0= (PL)/(P)tot
12
Q
Total protein
A
(P)tot=(PL) + (P)
13
Q
Total ligand
A
(L)tot=(PL)+(L)
