Lecture 7- Life and death of mRNAs in the cytoplasm Flashcards
Learning Objectives
Post-transcriptional control by RNA-binding
proteins and non-coding RNAs
Limited correlation of mRNA and protein levels
in cells
The fate of eukaryotic mRNAs is determined
through interaction with RBPs and ncRNAs
RNA export – how does the processed mRNA
get out of the nucleus?
Association with export receptors and
remodeling of mRNPs during nuclear export
The fate of eukaryotic mRNAs is determined
through interaction with RBPs and ncRNAs
Reasons for RNA localization?
RNA localisation
RNA transport in neurons to deliver mRNAs to
synapses for local protein synthesis
…more examples for mRNA localisation
Mechanism for the localisation of mRNAs
Cis-acting elements in the mRNA provide the
“ZIP” code for mRNA localization
mRNA delivery: ASH1 mRNA transport to
the bud-tip of dividing yeast cells
A RNA-binding protein (Khd1) represses translation
during mRNA transport
RNA cargo is release through phosphorylation
of transporter proteins at the destination
How to visualise mRNA localisation in real-time?
The fate of eukaryotic mRNAs is determined
through interaction with RBPs and ncRNAs
Two major modes of translational control
The fate of eukaryotic mRNAs is determined
through interaction with RBPs and ncRNAs
What is the life-expectancy of mRNAs and
proteins in human cells?
Degradation of eukaryotic mRNAs -
how does it work?
Structure of the core human exosome
Where is mRNA degraded?
mRNA stability controlled through interactions with
RNA-binding proteins e.g., cytokine mRNAs
Example: Signaling pathways regulate cytokine mRNAs
posttranscriptionally via AREs
Summary
Supplement 1: additional mRNA decay pathways
