Lecture 7 - AMD Flashcards
what are the hallmark findings of dry AMD?
drusen, RPE hyperpigmentation and RPE atrophy
what are the hallmark findings of wet AMD?
choroidal neovascularization, sub-retinal/sub-RPE fluid, and sub-retinal/sub-RPE blood
what is the risk of conversion from dry to wet AMD?
1-5% chance after 1 year and 13-18% chance after 3 years
who typically gets AMD?
12-30% over age 80, 89% white and 65% female
what is the biggest risk factor for AMD?
age = risk advances with increased age (2nd is race = caucasian because RPE pigmentation is protective)
what are other risk factors for AMD besides age and race?
family history (Tyr402His and Ala69Ser - part of complement factor H gene), smoking, UV exposure, systemic diseases (cardiovascular disease, obesity, HTN, high cholesterol)
how do you calculate the risk based on smoking?
pack years = if they smoked 1 pack a day for 1 year they have a 1 pack year smoking history (if they smoke 1/2 pack a day for 50 years they have a 25 year pack history)
what are the 5 layers of bruch’s membrane?
basement membrane of RPE, inner collagenous zone, elastic fiber layer, outer collagenous zone, basement membrane of the choriocapillaris
what happens to bruch’s with aging, what increases and what decreases?
increases = type 1 collagenm debris, filamentous material, glycosaminoglycans decreases = laminin, fibronectin and type 4 collagen
what is the pathophysiology of dry AMD?
problem with RPE, bruch’s or choriocapillaris = ultimately affects the photoreceptor function as a result of oxidative stress and inflammation
what happens during oxidative stress?
radiation (UV) and oxygen side effects (pro-oxidants) produce free radicals - damaged photoreceptors are taken in by RPE cells - RPE cells cannot handle amount and results in RPE damage
what happens during inflammation?
inflammation induces complement system - forms the membrane attack complex (MAC) - MAC injures RPE cells and endothelial cells of choriocapillaris
how does RPE damage occur?
damage to matrix metalloproteins (cannot get rid of byproducts like normal) - backed up byproducts get deposited between basal lamina of RPE and bruch’s = Drusen and RPE cells disorganize = atrophy and hypertrophy
what is the composition of drusen?
basal laminar deposits = lipid and collagen
basal linear deposits = phospholipid vesicles and granules
what are the sizes of drusen?
small less than 63um, intermediate between 64-124um, and large larger than 125um
what is the histology of drusen?
hyaline material accumulations and lipid rich
what is the result of hydrophobic and hydrophilic drusen?
hydrophobic = predispose RPE detachments hydrophilic = predispose formation of CNVM
what causes RPE atrophy?
photoreceptor death, large drusen push pigment away, perfusion abnormalities and decreased vascular density, flattening of RPE detachments and regression of confluent drusen
what is geographic atrophy?
may be the end stage of Dry AMD - will never develop wet (neo) the RPE is dead
what is the pathophysiology of wet AMD?
damage to RPE stimulates neo from choroid - new vessels contain fibroblasts (aka network or membrane) = VEGF and CNVM occur
what are the symptoms of dry AMD?
may be asymptomatic, gradual vision loss, central scotomas, and amsler grid defects
what are the signs of dry AMD?
hard/soft/confluent/calcified drusen - RPE hyperpigmentation - RPE hypopigmentation - RPE atrophy - geographic atrophy
what do hard drusen look like?
small (less than 64 microns), discrete and well demarcated
what do soft drusen look like?
larger than 64 microns, poorly defined borders, yellow-white
what do confluent drusen look like?
soft drusen that have coalesced - can cause focal RPE detachment
what do calcified drusen look like?
calcification of soft drusen - more white and refractile than soft