Lecture 7 + 8 Flashcards

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1
Q

DNA damage may result in which of the following

A

a. DNA double strand breaks
b. Increased levels of p53 protein
c. Increased activation of ATM
d. Increased activation of Chk1/Chk2

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2
Q

TP53 is a tumour suppressor gene encoding the p53 protein. What are the functions of the p53 protein?

A

a. Regulate DNA damage repair
b. Halt the cell cycle
c. Induce apoptosis
d. Function as a transcriptional activator and repressor

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3
Q

Are p53 mutated in a high percentage of cancers

A

Yes

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4
Q

How does p53 bind to DNA

A

As a tetramer

[A tetramer (tetra-, “four” + -mer, “parts”) is an oligomer formed from four monomers or subunits.]

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5
Q

Does p53 induces PUMA and APAF-1

A

Yes

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6
Q

Increased expression of MDM2 may result in

A

Degradation of p53

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7
Q

p53 is activated in response to DNA damage-Name
the kinases involved

A
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8
Q

How does the above (1.) relate to p53 protein levels?
ie what happens to p53 levels following DNA damage?

A
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9
Q

. p53 can block cell cycle progression at G1 and G2MHow? What are the main targets-what happens here

A
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10
Q

Mutant p53 acts in a dominant negative manner to
block wt p53-can you explain?

A
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11
Q

. Describe role of p53 in Apoptosis

A
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