Lecture 6- Histological Interpretation

Question 1

How should you examine tissue histologically?

Answer 1

What am I looking at?

Is it normal?

If no is it inflammatory or neoplastic?

If neoplastic is it benign or malignant

If malignant is it primary or secondary

Question 2

Use of immunohistochemistry?

Answer 2

If tissue known to have feature distinctive to it that can be stained then if the stain is used and a positive result attained then the tissue can be identified

Question 3

Histology vs cytology?

Answer 3

Cytology involves taking cells while histology involves taking tissue samples.

Question 4

Why can histology be more useful than cytology?

Answer 4

Histology allows you to appreciated the cell architecture

Question 5

Difference between appearance of lymphocyte, macrophage and eosinophil?

Answer 5

Lymphocyte has a large purple nucleus tha takes up most of the cell. Macrophage has a fluffy cytoplasm and is quite a big cell. Eosinophil looks like a tomato with sunglasses

Question 6

How to tell histologically if inflammation is acute or chronic?

Answer 6

Acute if bundles of neutrophils, chronic if bundles of lymphocytes

Question 7

How to tell benign from malignant histologically?

Answer 7

Malignant= enlarged nuclei, bigger cells, mitotic figures, hyperchromatism, disorganisation of cells

Question 8

Give an example f a malignant cell type?

Answer 8

Reed Sternberg cell in Hodgkin’s lymphoma..

Only cell that will look like an owls eye

Question 9

How are cancers graded?

Answer 9

Grading only applies to malignant tissue.

Well differentiated looks a lot like parent tissue and will be grade 1

Poorly differentiated looks very unlike parent tissue and will be graded 3/4