Lecture 6 - Cell Biology & Protein Purification Flashcards
1
Q
What are the features of the peptidoglycan layer?
A
- The PG layer is mechanically tough.
- It is held together by covalent bonds.
- Must be artificially damaged to release proteins from the cell.
- Most bacteria require an intact PG layer to survive.
- Also a target for antibiotics.
2
Q
What are some of the components needed to partially reproduce ‘physiological’ conditions?
A
Almost all “buffers”
* Buffer molecule (phosphate, Tris)
* Salt (NaCl, KCI)
Popular additives
* Other salts (MgC12, CaC12)
* Detergents
* Protease inhibitors
* Nuclease inhibitors
3
Q
What are the different types of chromatography?
A
Affinity chromatography
Ion-exchange chromatography
Hydrophobic-interaction chromatography
Gel-filtration chromatography
