Lecture 5 Sec 61a

Question 1

stuff you missed on protein insertion into the membrane

Answer 1

crytallisation of PCC channel
the nascent polypeptide inserts as a loop into the PCC channel with the hydrophobic signal sequence intercalated into the open lateral gate

A hydrophilic peptide can translocate through the Sec complex with an essentially
closed lateral gate and an only slightly rearranged central channel

Membrane insertion of a hydrophobic domain seems to occur with
the Sec complex opening the proposed lateral gate while rearranging
the plug to maintain an ion permeability barrier.

Question 2

how are these are targetted and inserted into the membrane

Answer 2

S-methionine labeled b1AR was translated in
reticulocyte lysate (RRL) in the absence or presence
of HEK293-derived rough microsomes (hRM)
from wild-type (WT) or DEMC6

Question 3

what are the main proteins involved

Answer 3

w