Lecture 5: Fundamentals of Mass Spectrometry: Hardware Flashcards
what are the components of the mass spectrometer?
inlet, source, analyser, ion detector, data system
what does the ion source produce?
ions of the analytes
what does the analyser do?
analyzer manipulates the ions according to their mass-to-charge (m/z) ratio.
what does the detector system do?
The detector system detects the ions and records the relative abundance of the ion species.
If the ion source for gas sample what can it be?
Electron Ionization (EI) Chemical Ionization (CI)
what the common liquid ion source?
Electrospray Ionization (ESI)
what happen with gas sample ionisation?
Not just an ion being formed there will be a charge.
what are two types of ionisation?
hard and soft
what are hard ionisation techniques ?
Electron Ionisation (EI)
what produces more fragments hard ionisation techniques soft?
hard produces more
soft ionisation techniques are what?
Chemical ionisation (CI), Fast Atom Bombardment (FAB), Electrospray ionisation (ESI)
what better for determining molecular mass, soft or hard?
soft ionisation techniques
How does electro ionisation work?
- Molecules are ionized during collision with a beam of high energetic electrons
- Electrons are “boiled off” in a filament
what energy is the beam in electron ionisation?
(typical 70 eV energy)
EI is great for finding the?
identification of structures
what does the high internal energy during EI equal?
Due to high internal energy high degree of fragmentation (which occurs IN ion source)
with EI what do u get?
mass spectrum