Lecture 5 Flashcards by Julia Toronczak | Brainscape

Q

RNA-seq

A

RPKM/FPKM) (genome-wide steady-state RNA)

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Q

GRO-seq

A

genome wide nascent RNA

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Q

ChIP-seq

A

locations of accessible antigens in chromatin

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Q

Heat maps

A

simultaneous display of many loci

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Q

MNase-seq

A

locations of canonical nucleosomes

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Q

MPE-seq

A

locations of noncanonical chromatin particles

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Q

paired-end sequencing

A

both ends of DNA fragments

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Q

DNase-seq/ATAC-seq

A

genome-wide chromatin accessibility

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Q

3 C, Hi-C

A

chromatin looping; 3D organization of chromatin

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Q

RNAi

A

RNA interference– specific depletion of RNA’s and proteins

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Q

CRISPR-cas 9

A

gene targetting

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Q

MS2 and PP7

A

real-time detection of RNA in cells

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Q

degration of proteins in cells

A

Trim-Away, AID

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