Lecture 5 Flashcards
1
Q
What is the effect of the anneal ing temp on PCR products?
A
as temp increases- the primers will bind less effectively=less product made
as temp decreases- the primers may bind alternative sites- non specific products
2
Q
Ligation
A
covalent join vector and insert DNA
3
Q
Transform
A
introduce ligation mixture into host cells for replication of DNA. E.coli is the hose we will use
4
Q
Formula for ligation
A
insert size/vector size * insert/vector= ng insert/ ng vector
5
Q
Ligation and transformation controls
A
look at chart