Lecture 5

Question 1

What is eukaryotic expression controlled by?

Answer 1

Protein-binding transcription-control regions that are located at various distances from the transcription start site

Question 2

What are the control regions called that are close to the transcription initiation site?

Answer 2

Promoter-Proximal Regions

Question 3

What are the control regions called that are more distal from the transcription start site?

Answer 3

Enhancers

Question 4

What are both Promoter-Proximal Regions and Enhancers?

Answer 4

Gene and cell type specific regulatory elements on DNA

Question 5

What are transcription activators and repressors?

Answer 5

Are modular proteins that contain a DNA binding domain and one or more activation or repression domains

Question 6

What technique can be used to find gene regulatory elements on DNA?

Answer 6

Linker-scanning reporter gene technique

Question 7

How would you obtain a reporter gene?

Answer 7

Buy a plasmid at the store that contains a reporter gene

Question 8

What is a reporter gene?

Answer 8

A reporter gene is a gene that encodes for an enzyme (in this case, it is tk)

Question 9

What did the tk ORF (open reading frame) not contain?

Answer 9

A promoter

Question 10

What do you do upstream of the tk (ORF)?

Answer 10

Clone a large piece of DNA that you know is controlling YOUR gene of interest (not tk)