Lecture 4 - DNA detection and manipulation

Question 1

After 3 cycles of PCR how many double stranded target DNA sequences exist?

Answer 1

8

Question 2

Which of the following is/are required for the polymerase chain reaction to occur?

Answer 2

Target DNA, Nucleotides, Taq polymerase

Question 3

In Sanger sequencing what is the difference between dNTPS and ddNTPS?

Answer 3

ddNTPs lack the 3’OH group of their sugar

Question 4

Which of the following is NOT a benefit of real-time PCR of standard PCR?

Answer 4

??

Question 5

The CRISPR Cas9 system consists of all the following:

Answer 5

Cas9 gene, a guide RNA semi-palindromic repeats

Question 6

Genome editing via the CRISPR_ Cas9 system cannot generate:

Answer 6

repeat expansion

Question 7

What is found in a plasmid vector?

Answer 7

A drug resistant gene, a multi cloning system, a replication origin

Question 8

In next-generation sequencing by synthesis NGS which is a trait feature of the sequencing process?

Answer 8

Incorporation of fluorescently labelled dideoxynucleotides