Lecture: 4-6 Diversity of Bacteria Flashcards

1
Q

What are firmicutes?

A
  • a type of bacteria
  • classically gram positive bacteria
  • mostly organotrophs
  • range from obligate aerobes to obligate anaerobes
    examples: lactic acid bacteria and endospore formers
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2
Q

What are lactic acid bacteria?

A
  • an example of firmicutes
  • they are aeroteolerant anaerobes
  • convert simple sugars into lactic acid
  • no use of electron transport chain, no external electron acceptor
    location: found in carbohydrate rich areas : plant decay, mouth/gastrointestinal tract (vagina), and the dairy / pickling industry (adds flavour, acids inhibit other microbes)
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3
Q

What do lactic acid bacteria (firmicutes) breathe?

A
  • they are aerotolerant anaerobes, so they do not breathe oxygen but they are ok with it in their environment
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4
Q

What are endospore formers? provide an example

A
  • eg; bacillus
  • mostly found in soil/sediment, endospores are a dormancy stage (resistant to heat, UV, desiccation, etc) - some stay viable for decades
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5
Q

What are endospores specifically? What do they surround?

A
  • endospores have a thick cortex of modified peptidoglycan
  • cortex surrounds the core, which holds the nucleoid and inactive cytoplasm (low water content)
  • the DNA are bound to protective, small, acid soluble proteins!
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6
Q

What is the process of sporulation?

A
  • sporulation is when the spore is actually released
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7
Q

What is responsible for the pathogenicity of pathogenic endospore formers?

A
  • partly due to extremely potent toxins
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8
Q

Describe actinobacteria

A
  • another form of bacteria
  • gram positive cell architecture
  • organotrophs: mostly aerobic
  • abundant in soil
  • mostly filamentous
  • some are unicellular
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9
Q

Describe filamentous bacteria

A
  • filaments indefinite length (branch and extend as mycelium, many copies of genomes)
  • release thick walled spores called arthrospores or exospores
  • resist desiccation, different from the endospores of firmicutes (eg; produced by multiple fission), have reproduction and function in dispersal
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10
Q

How do filamentous actinobacteria gain their nutrients?

A
  • acquire nutrients by growing substrate mycelium
  • then aerial mycelium grow and release arthrospores
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11
Q

Do actinobacteria have multiple copies of genes? Why?

A
  • filamentous Bacteria have dozens of copies of chromosomes which can then be released as spores from the arthrospores (helps in reproduction and dispersal)
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12
Q

Describe streptomyces?

A
  • a form of actinobacteria
  • grow on (break down) durable polysaccharides (ligin, chitin)
  • important in soil decomposition
  • also source of antibiotics
    ~50% of therapeutic antibiotics come from streptomyces
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13
Q

Describe mycobacterium?

A
  • a type of actinobacteria
  • mostly found in soil but some pathogens (eg; tuberculosis)
  • slow growth, highly resistant to antibiotics, does not stain gram positive
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14
Q

Describe proteobacteria

A
  • another form of bacteria
  • gram negative
  • range from largest prokaryote to smallest parasite
  • metabolically diverse: organotrophs, lithographs, phototrophs,
  • major phylogenetic groups: alpha, gamma, beta - proteobacteria
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15
Q

Describe enteric bacteria

A
  • an organotrophic (gamma) proteobacteria
  • 40 different genera
  • facultative anaerobes: can respire via nitrate reduction, and ferment simple sugars
  • animal hosts (especially intestinal tract)
  • many are (or can be) pathogenic
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16
Q

What is a MacConey Medium?

A

an example of a medium that differentiates bacteria based on their fermentation ability

eg; can distinguish between lactose fermenting bacteria (acidic turns pink) and non lactose fermenting

17
Q

Describe E.Coli

A
  • an example of proteo bacteria
  • likely the best studied bacteria (fundamental cell biology and biochemistry), and a mainstay for molecular cloning
  • mainly reside in animals intestines
  • indictor organism for fecal contamination of water
18
Q

Describe Pseudomonads

A
  • an example of proteobacteria
  • a facultative anaerobe
  • limited fermentation abilities, uses many alternatives as electron acceptors including denitrification
  • pseudomonas aeruginosa
  • found on soil but also pathogenic
  • burn wounds, lungs (esp. CF)
19
Q
A