lecture 4 Flashcards
what are the steps in transcription?
- initiation
- elongation
- termination
what supports transcription?
- transcription regulators
- transcription factors
what is transcription?
copy the DNA into RNA
how many RNA polymerases do eukaryotes have?
3
how many RNA polymerases fo bacteria have?
1
what is the structure of bacteria RNA polymerase?
5 subunits
- 400-450 kDa
what is the function of RNA polymerases?
reading and transcribing a gene sequence into an RNA product
is RNApol a conserved structure?
RNA polymerase general architacture and catalytic function is conserved across the three domains of life
what is the action of bacterial RNA pol?
- bacterial RNAP has a crab-cpaw appearance that opens and closes during transcription
- the incoming dsDNA goes into the cleft, becomes unwound and is transcribed into a strand of RNA
- the exiting DNA comes out in a perpendicular direction
what is the structure of the core of RNA polymerase?
parts of the beta and beta’ and the two alpha subunits
what is the structure of the jaw-lobe of RNA polymerase?
parts of the beta subunit
what is the role of sigma in bacterial RNA polymerase?
participates in recognizing the promoter and initiate transcription
where does the sigma factor bind on bacterial RNA polymerase?
to the cleft
what happens in the initiation stage of transcription?
RNAP binds to the promoter, near the beginning of a gene transcription bubble
what happens in the elongation stage of transcription?
RNAP reads the template strand one base at a time
RNAP buikds a complementary RNA molecule