lecture 3 - non coding RNA Flashcards
what does the expression profile of sets of different genes vary in
different cell types
what does the expression level of sets of genes vary in
different stages and time points
different signal responses
why do genes need to be regulated
control physiological processes
maintain healthy function of specialised cells
instruct organism development
what changes do genes respond to
environmental stress
defence against pathogens
domestic issues
what are the gene regulation mechanisms
initiation
suppression
inactivation
what should activation of gene expression involve
selective disruption of the folded structure exposing those genes genes that need to be switched on
what do transcription factor act as
a mediator between RNA polymerase and DNA strand
what is an example of a transcription factor
NFATC1-DNA
what happens after the Tf binds to the promoter region
the DNA they interact with other bound Tf and recruit RNA
what must the DNA be for the gene to be expressed
accesible
what is the process for DNA to be accessible called
Chromatin remodelling
what must the DNA be accessible to
transcription factors and RNA polymerises
what are 3 ways of chromatin remodelling
nucleosome remodelling
covalent modification of histones within the nucelosome
histone variants may replace one or more of the core histones
where does the. multiprotein complex get their energy from to remodel
energy of ATP hydrolysis to mobilise and restructure nucleosomes
what does diversity in cofactors allow
selective interaction of the chromatin remodelers complexes with other regulatory proteins that bind to specific DNA sequences
