Lecture 3

Question 1

organisms with more DNA (name 2)

Answer 1

newt, fern, bean, lily, amoeba

Question 2

polarity / directionality of DNA

Answer 2

3’ end

• hydroxl end
• end where new bases get added

5’ end

• phosphate end
• terminus of the strand

Question 3

template

Answer 3

original strand of DNA

Question 4

complementary

Answer 4

new strand of DNA

Question 5

__ bonds G-C

Answer 5

3 hydrogen bonds

Question 6

__ bonds A-T

Answer 6

2 hydrogen bonds

Question 7

DNA melting temp equation

Answer 7

tm ~ 64.9 + 0.41(%GC) - (500 / Length of DNA)

Question 8

DNA melting temp range

Answer 8

~90 - 95 C

Question 9

DNA hybridization temp range

Answer 9

~40 - 60 C

Question 10

charge of DNA

Answer 10

has a negative charge due to phosphate-deoxyribose backbone

Question 11

How is a cDNA library produced?

Answer 11

Polymerase Chain Reaction (PCR)

Question 12

cDNA

Answer 12

library of DNA that only codes for genes (no introns)

Question 13

reverse transcriptase

Answer 13

key enzyme used to produce the cDNA library without introns – synthesises DNA from mRNA strand

Question 14

Northern Blot

Answer 14

type of gel electrophoresis – detection of a specific mRNA in a band

Question 15

Southern Blot

Answer 15

type of gel electrophoresis – detection of specific DNA in a band