Lecture 25 viral outbreak and response FLIP Flashcards
what specimens for the outbreak would be collected
- blood
- urine
- faeces
- sputum
- CSF
- can do an MRI
what does it indicate if lymphocytes are in the CSF
- if there are lymphocytes in the CSF this indicates viral infection
what methods to you use to identify the cause of the outbreak
- EM - direct viewing
- culture the virus if possible
- serology
- sequencing of virus
PCR / RT-PCR (consensus PCR) - PCR is quick and effective
what type of PCR should you use in an unknown outbreak
you should use consensus PCR as it allows us to detect novel viruses quickly
- other PCRs are often very virus specific
infectious agent was a borna virus
what type of genome is borna virus?
what is borna virus associated with?
how is it commonly transmitted
negative ssRNA
associated with behavioural changes and changes in the brain
mainly transmitted through enteric route
how would you rapidly diagnose a new virus?
RT-PCR is used for rapid diagnosis
How do you determine a virus sequence / if the virus is new etc.
- whole genome sequencing
- will allow you to determine if the virus is recombinant or where the virus originated from etc
what would an epidemiologist do in an outbreak of a new viral infection?
- look at how the virus is being transmitted
- would gather information about the symptoms of the virus including those that are asymptomatic and symptomatic
who is most at risk in a new infection?
health care workers
how would a vaccine to a new virus be made
if the virus can be cultured:
- live attenuated
- inactivated
- VLPs
if virus cant be cultured
- use recovered persons Ab’s to make a therapeutic vaccine to prevent disease worsening
- may also be used to prevent it
other than vaccines how can we treat a new virus
- with antiviral drugs
what viruses can be found in the urine?
flavis - dengue
ebola virus
