Lecture 25: Microbiology for Clinic

Question 1

2 gram stain results

Answer 1

1 from actual sample

1 from what is grown in culture

Question 2

T/F: Swabs are the worst.

Answer 2

True. Small specimen volume. Lots of problems with getting adequate sample and culturing.

Question 3

Type of collection container for aspirated fluid specimens

Answer 3

Anaerobic vial w/ rubber septum

Question 4

Which of the following is FALSE about MALDI-TOF:
A. Fast
B. Expensive
C. Any organism w/ unique protein mass spectrum can identify
D. Looks at RNA components of an organism

Answer 4

B, D

D. Looks at ribosomal components

Question 5

UTIs are cinically significant when culture grows how much?

Answer 5

> 100,000 CFU/ml

Question 6

Positive LR =

Answer 6

odds of disease increase when test is positive

Ugh…EPCP here to haunt us again…

Question 7

Negative LR =

Answer 7

odds of disease decreases when test is negative

Question 8

T/F: Only order a throat culture if strep antigen test is negative.

Answer 8

False. Always order a throat culture along with strep antigen test.

*Slightly contradictory to other lectures but… shrugs shoulders Just learn how to read the test question writer’s mind. Easy peasy.

Question 9

Resistance to what 2 abx is cause for concern (emergency!)

Answer 9

Cefriaxone and Meropenem

Question 10

Features of Carbapenem Resistant Enterobacteriaceae (2)

Answer 10

Resistant to spectrum cephalosporins

Carbapenem MICs elevated