Lecture 22: Experimental Methods in Renal Physiology

Question 1

How can you study nephrons in vitro?

Answer 1

Dissection

Question 2

How can you measure fluid flow in vivo?

Answer 2

use micropuncture technique. use two micropipettes, one to perfuse, one to aspirate (collect for analysis)
use wax block to prevent normal flow so that you can specify the composition of the fluid