Lecture 21 - Cancer Flashcards
What is a tumour?
A group of over-poliferating cells
What is a key characteristic of benign tumours?
They’re not able to invade neighbouring tissues and therefore are not cancerous
What is cancer?
A malignant tumour which can invade nearby tissues
What is a metastatic tumour?
Cancer that spreads to secondary sites - via travel of cancer cells through lymph or blood system
Cancer classifications: Carcinomas
- Arise from epithelial cells
- Most common and most dangerous cancers in humans
Cancer classifications: Sarcomas
Arise from connective tissue or muscle
Cancer classifications: Leukemias and lymphomas
Arise from white blood cells
What can general cancer classifications be further characterized by?
Specific cell type affected
- E.g., melanoma, small-cell lung carcinoma
What are the original theories on the origins of cancer?
- Chemicals and other agents that damage DNA (mutagens) as causes of cancer
- Viruses as causes of cancer
Gene mutations as causes of cancer: 1775
First study linking chemical exposure to cancer
- Observation that chimney sweeps have high incidences of cancer
Gene mutations as causes of cancer: 1918
First experimental evidence that chemical agents can cause cancer in animals
- Tar exposure leads to increased cancer incidence (in rabbits)
Gene mutations as causes of cancer: 1920s
Humans that work with radiation have high incidence of cancer
Gene mutations as causes of cancer: 1930s
Chemicals and radiation result in mutations in DNA
- Carcinogens = mutagens
Gene mutations as causes of cancer: 1960s
In vitro transformation of normal cells to cancer cells using chemical mutagens
What happens to normal cells grown in culture from a monolayer?
- Cells that lose contact with plate surface undergo apoptosis - anchorage dependence
- Cells divide about 30 times before replication-induced senescence or they stop dividing after reaching a uniform monolayer - contact inhibition
- Cells require added serum to survive and grow
What happens when normal cells are turned into cancer cells in vitro by adding chemicals and radiation?
- Loss of anchorage dependence
- Loss of contact inhibition
- Ability to divide indefinitely - immortality
- Reduced requirement for added serum
What was the first characterized tumour virus?
Rous sarcoma virus - RSV
What did characterized RSV lead to?
The model that cancer is caused by infectious agents
What kind of virus is RSV?
Retrovirus
In the 1960s, what was discovered about RSV?
Leads to
- Loss of anchorage dependence
- Loss of contact dependence
- Ability to divide indefinitely - immortality
- Reduced requirement for added serum
Viruses as causes of cancer: 1910
RSV virus causes chicken sarcoma
Viruses as causes of cancer: 1960s
Purified RSV can cause cell transformation in vitro
Viruses as causes of cancer: 1960s
Several unrelated viruses found to also cause cancer
Viruses as causes of cancer: 1968
Tumour virus DNA integrates into host chromosomal DNA
Viruses as causes of cancer: 1974
RSV transformation ability linked to a single viral gene - later named src
At what rate does murine leukemia virus (MLV) cause cancer compared to RSV?
MLV causes slow onset of cancer whereas RSV causes cancer quickly
What does the RSV src gene do?
Confers transformation ability to cells