Lecture 2 Flashcards

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1
Q

Where are normal bacterial flora acquired

A

During birth

Shortly after birth

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2
Q

Do the bacteria stay the same throughout our life

A

No they change

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3
Q

What can the bacteria present tell us

A

Age

Nutrition status

Environment of individual

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4
Q

Breastfed children will hve mainly what 2 bacteria in their GIT

A

Lactic acid streptococcus

Lactobacilli

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5
Q

What is the commets bacteria on our skin

A

Staphylococcus eperdermidis

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6
Q

Our skin changes in puberty what can lead to increased levels of acne

A

The bacteria prioionibacterium acnes

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7
Q

Name 2 bacteria associated with the nose and mouth

A

Streptococci

Gram negative cocci

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8
Q

There are 5 distributions of bacterial phyla what are they

A

Fermicutes

Bacteriodetes

Actionbacteria

Proteobacteria

Other phyla

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9
Q

How many bacterial phyla are there and how many are dominant

A

More than 50

4 dominate

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10
Q

What species is there a lack of

A

Archaeal species

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11
Q

Where is the majority of bacteria found in the body

A

Inside the gut

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12
Q

The bacteria in the gut what do they contribute to

A

Harvest energy from food

Normal immune function

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13
Q

What is associated with changes in the microbiome

A

Bowel disease

Obesity

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14
Q

Bacteriodes and fornicate a constitute how much phyla in the gut

A

90%

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15
Q

What will the bacteria have in common despite being a different species

A

Very similar function

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16
Q

What sort of gut metabolism do the gut microbiota do

A

Carbohydrate and amino acid metabolism

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17
Q

What is the gut considered as

A

Macro ecosystem

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18
Q

When do microbial communities stop going through changes

A

When there is a stable community established

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19
Q

How do people in different populations get different gut bacteria

A

They are exposed to different environmental exposure, adequate sanitation, diet and antibiotic use

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20
Q

Children in Africa on a high fibre diet have levels of

A

Prototella

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21
Q

What does provotella do

A

Degrades cellulose and xylans, increase short chain fatty acids and maximal energy extractions

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22
Q

What do children in IS have more of than the children of Africa

A

Bacteriodes

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23
Q

How do we get bactericides from

A

Long term diet rich is animal protein and sat fat

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24
Q

How do we get provotella

A

Carbs and sugars

25
Q

The human gut microbiome can be divided into 3 enterotypes

A

Bacteriodes

Provotella

Ruminococcus

26
Q

Who has the most types of microbe in their gut
Lean people
Fat people

A

Leaner

27
Q

Normal flora has how many human cells to bacteria

A

Cells 10’13

Bacteria 10’14

28
Q

Obese and lean people how do they differ in regards to their microbiota

A

Obese have fewer microbiota in their gut

29
Q

Name 2 species of bacteria found in the stomach that are acid resistant

A

Lactobacilli

Streptococci

30
Q

What bacteria are found in the ileum

A

Streptococci
Lactobacilli
Enteribacteriaceae
Bacteriodes

31
Q

What are most of the bacteria in the body are they aerobic or anaerobic

A

Anaerobes

32
Q

How many bacteria are found in the gut

A

57

33
Q

The microbiome of the gut what are they dependent of

A

Complex sugar

34
Q

Why are gut bacteria dependent on fermentation

A

For energy needs

35
Q

What do gut bacteria convert sugars into

A

Short chain fatty acids

36
Q

What uses acetate

A

Heart
Muscle
Brain cells

37
Q

What uses propionate

A

Liver - gluconeogenesis

38
Q

What uses butyrate

A

Enterocytes

39
Q

What do some bacteria provide in the gut

A

Amino acids

Vitamins

40
Q

What do bacteria also degrade

A

Xenobiotics - food additives

41
Q

What can’t human enzymes degrade most of

A

Complex carbohydrates

Plant polysaccharides

42
Q

So how are non digested carbohydrates broken down

A

They are fermented in the colon by microbiota for energy

43
Q

What are considered to be as end products

A

Acetate
Proprionate
Butyrate

44
Q

What is butyrate

A

Colonic epithelial

45
Q

What is acetate and propionate

A

Peripheral tissue

46
Q

How do products of microbial metabolism affect humans

A

They act as signalling molecules and affect host metabolism

47
Q

What process uses acetate and proprionate

A

Used in the liver by

Lipogenesis

Gluconeogenesis

48
Q

What type of bacteria is found in urethra

A

Staph epidermidis

Strep faecalis

49
Q

What bacteria are found before puberty

A

Staphylococci

Streptococci

E. coli

50
Q

After puberty what bacteria is present

A

Lactobacillus aerophilus

51
Q

What does lactobacillus aerophilus do in the vaginal area

A

Ferments glycogen

Maintains acidic ph

Prevents overgrowth of other vaginal organisms

52
Q

How does thrush come about

A

Acidic ph is lost

53
Q

How does antibiotics affect flora

A

Can reduce it to a minimum

54
Q

What can normal flora prevent

A

Prevents colonisation

55
Q

What do gut bacteria release

A

Antibacterial agents - bacteriocins and colicins

56
Q

Name disadvantages of normal flora

A

Can spread to sterile regions of the body if:

Skin is broken

Organisms get into urethra

57
Q

Symbiotic relationships can be divided into

A

Commensalism

Parasitism

Mutalism

58
Q

Benefits of commensalism

A

Prevent colonisation in intestinal flora

Produce metabolites used by host

59
Q

What can a host provide to a virus

A

Basic building blocks

Transcriptions and translation machinery

Energy requirement