Lecture 2 Flashcards

1
Q

Where are normal bacterial flora acquired

A

During birth

Shortly after birth

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2
Q

Do the bacteria stay the same throughout our life

A

No they change

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3
Q

What can the bacteria present tell us

A

Age

Nutrition status

Environment of individual

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4
Q

Breastfed children will hve mainly what 2 bacteria in their GIT

A

Lactic acid streptococcus

Lactobacilli

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5
Q

What is the commets bacteria on our skin

A

Staphylococcus eperdermidis

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6
Q

Our skin changes in puberty what can lead to increased levels of acne

A

The bacteria prioionibacterium acnes

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7
Q

Name 2 bacteria associated with the nose and mouth

A

Streptococci

Gram negative cocci

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8
Q

There are 5 distributions of bacterial phyla what are they

A

Fermicutes

Bacteriodetes

Actionbacteria

Proteobacteria

Other phyla

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9
Q

How many bacterial phyla are there and how many are dominant

A

More than 50

4 dominate

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10
Q

What species is there a lack of

A

Archaeal species

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11
Q

Where is the majority of bacteria found in the body

A

Inside the gut

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12
Q

The bacteria in the gut what do they contribute to

A

Harvest energy from food

Normal immune function

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13
Q

What is associated with changes in the microbiome

A

Bowel disease

Obesity

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14
Q

Bacteriodes and fornicate a constitute how much phyla in the gut

A

90%

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15
Q

What will the bacteria have in common despite being a different species

A

Very similar function

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16
Q

What sort of gut metabolism do the gut microbiota do

A

Carbohydrate and amino acid metabolism

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17
Q

What is the gut considered as

A

Macro ecosystem

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18
Q

When do microbial communities stop going through changes

A

When there is a stable community established

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19
Q

How do people in different populations get different gut bacteria

A

They are exposed to different environmental exposure, adequate sanitation, diet and antibiotic use

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20
Q

Children in Africa on a high fibre diet have levels of

A

Prototella

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21
Q

What does provotella do

A

Degrades cellulose and xylans, increase short chain fatty acids and maximal energy extractions

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22
Q

What do children in IS have more of than the children of Africa

A

Bacteriodes

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23
Q

How do we get bactericides from

A

Long term diet rich is animal protein and sat fat

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24
Q

How do we get provotella

A

Carbs and sugars

25
The human gut microbiome can be divided into 3 enterotypes
Bacteriodes Provotella Ruminococcus
26
Who has the most types of microbe in their gut Lean people Fat people
Leaner
27
Normal flora has how many human cells to bacteria
Cells 10'13 | Bacteria 10'14
28
Obese and lean people how do they differ in regards to their microbiota
Obese have fewer microbiota in their gut
29
Name 2 species of bacteria found in the stomach that are acid resistant
Lactobacilli | Streptococci
30
What bacteria are found in the ileum
Streptococci Lactobacilli Enteribacteriaceae Bacteriodes
31
What are most of the bacteria in the body are they aerobic or anaerobic
Anaerobes
32
How many bacteria are found in the gut
57
33
The microbiome of the gut what are they dependent of
Complex sugar
34
Why are gut bacteria dependent on fermentation
For energy needs
35
What do gut bacteria convert sugars into
Short chain fatty acids
36
What uses acetate
Heart Muscle Brain cells
37
What uses propionate
Liver - gluconeogenesis
38
What uses butyrate
Enterocytes
39
What do some bacteria provide in the gut
Amino acids | Vitamins
40
What do bacteria also degrade
Xenobiotics - food additives
41
What can't human enzymes degrade most of
Complex carbohydrates | Plant polysaccharides
42
So how are non digested carbohydrates broken down
They are fermented in the colon by microbiota for energy
43
What are considered to be as end products
Acetate Proprionate Butyrate
44
What is butyrate
Colonic epithelial
45
What is acetate and propionate
Peripheral tissue
46
How do products of microbial metabolism affect humans
They act as signalling molecules and affect host metabolism
47
What process uses acetate and proprionate
Used in the liver by Lipogenesis Gluconeogenesis
48
What type of bacteria is found in urethra
Staph epidermidis Strep faecalis
49
What bacteria are found before puberty
Staphylococci Streptococci E. coli
50
After puberty what bacteria is present
Lactobacillus aerophilus
51
What does lactobacillus aerophilus do in the vaginal area
Ferments glycogen Maintains acidic ph Prevents overgrowth of other vaginal organisms
52
How does thrush come about
Acidic ph is lost
53
How does antibiotics affect flora
Can reduce it to a minimum
54
What can normal flora prevent
Prevents colonisation
55
What do gut bacteria release
Antibacterial agents - bacteriocins and colicins
56
Name disadvantages of normal flora
Can spread to sterile regions of the body if: Skin is broken Organisms get into urethra
57
Symbiotic relationships can be divided into
Commensalism Parasitism Mutalism
58
Benefits of commensalism
Prevent colonisation in intestinal flora Produce metabolites used by host
59
What can a host provide to a virus
Basic building blocks Transcriptions and translation machinery Energy requirement