Lecture 2 Flashcards
When is oocyte pick-up (OPU) typically scheduled after the trigger shot?
36 hours
Where must oocyte pick-up (OPU) be performed?
A licensed area
Name three healthcare professionals involved in the OPU procedure.
Any three of the following:
Clinician
Anesthetist
Anesthetic Nurse
Embryologist
List three essential considerations for an embryologist during an OPU procedure.
- Replicating in-vivo conditions (temperature and pH)
- Speed and accuracy in handling COCs
- Witnessing for patient identification and labeling
What are COC’s
Cumulous oocyte complex
How quickly should sperm be removed from the ejaculate for preparation?
Within 1 hour
Name two methods used for sperm preparation.
Density gradient and centrifugation8
Swim-up
When should oocytes be inseminated after the trigger shot?
38-42 hours post-trigge
What is the typical sperm concentration used for conventional IVF insemination?
80,000 to 120,000 motile sperm
Describe the process of intracytoplasmic sperm injection (ICSI).
Oocytes are stripped of their cumulus complex, assessed for maturity, and a single sperm is injected into each mature (MII) oocyte.
What enzyme is used to assess oocyte maturity for ICSI?
Hyaluronidase
When is the fertilization check performed after insemination?
17 ±1 hours post-insemination
What is the outcome for the majority of embryos?
Discarded in biohazard waste
Why is it necessary to remove cumulus cells before fertilization check in conventional IVF?
Although sperm will have broken down most of the cumulus during insemination, narrow pipettes are still needed to remove remaining cumulus cells for oocyte assessment
Match the following IVF procedures with their typical timing:
1.Oocyte collection
2.Insemination (IVF or ICSI)
3.Fertilization check
a) +17 hpi (hours post-insemination)
b) +36 hpt (hours post-trigger)
c) +38-42 hpt (hours post-trigger)
1.b
2.c
3.a
How many hours post trigger does the insemination need to happen?
38-42
