Lecture 1b Microbial Growth & Metabolism

Question 1

Final products of glycolysis…

Answer 1

• 4 ATP (2 Net)

- 2 NADH

Question 2

Hexokinase function….

Answer 2

Step 1: Glycolysis (Uses ATP)

• Add Pospphate at the 6th carbon of glucose
• Creates Glucose-6-Phosphate

Question 3

Phosphoglucose Isomerase function…

Answer 3

Step 2: Glycolysis

• Creates an isomer
• Converts Glucose-6-Phosphate to Fructose-6-Phosphate

Question 4

Phoshofructokinase function….

Answer 4

Step 3: Glycolysis (Uses ATP)

• ADDS phosphate group to 1st carbon of Fructose-6-Phosphate
• Creates Fructose-1,6-Biphosphate

Question 5

Adolase function….

Answer 5

Step 4: Glycolysis

• Fructose-1,6-Bisphospate —> two 3 carbon molecules

1. Dihydroxyacetone phosphate (DHAP)
2. Glyceraldehyde-3-Phosphate

Question 6

Triphosphate Isomerase

Answer 6

Step 5: Glycolysis

• Maintains balance of ATP production & usage
• Dihydroxyacetone- STOPS glycolysis
• Glyceraldehyde-3-Phosphate- More ATP

Question 7

Glyceraldehyde Phosphate Dehydrogenase function….

Answer 7

Step 6: Glycolysis (Uses NAD + Pi)

• 1,3-bisphosphoglycerate (1,3,BPG)
• Produces 2 NADH + H+
• Donates Phosphate group Picks up H ion

Question 8

Phosphoglycerate Kinase function….

Answer 8

Step 7: Glycolysis (Uses ADP)

• Happens 2X
• Picks up phosphate to creates ATP
• Produces 3-Phosphoglycerate

Question 9

Phosphoglyceromutase function…

Answer 9

Step 8: Glycolysis

• 3-Phosphoglycerate ——->2-Phosphoglycerate

Question 10

Enolase function….

Answer 10

Step 9: Glycolysis (Releases H2O)

• Produces Phospoenolpyruvate

Question 11

Pyruvate Kinase function….

Answer 11

Final Step: Glycolysis (Uses ADP)

• Phosophenolpyruvate —–> Pyruvate
• Produces ATP
• Pyruvate w/O2—-> Krebs Cycle
• Pyruvate w/o O2——-> Lactic acid

Question 12

What steps in glycolysis Use ATP?

Answer 12

• Hexokinase
  (Glucose—-> Glucose-6-Phosphate)

-Phosphofructokinase
(Fructose-6-Phosphate–> Fructose-1,6 Bisphosphate

Question 13

What Steps in Glycolysis use ADP?

Answer 13

-Phosphoglycerate kinase
(1,3-Bisphosoglycerate-> 3-Phosphoglycerate)

• Pyruvate Kinase
  ( Phosphophenolpyruvate—> Pyruvate)

Question 14

What steps in glycolysis realease H2O?

Answer 14

Enolase

2-Phosphoglycerate—-> Phosphenolpyruvate

Question 15

Final products of Oxidative Decarboxylation….

Answer 15

Done TWICE
2 Pyruvate—>

• 2 Acetyl-CoA
• 2 CO2
• 2 NADH

Question 16

In Oxidative Decarboxylation, Acetyl-CoA fxn…

Answer 16

A carrier molecule for the 2 Carbon fragment

Question 17

Where does glycolysis occur?

Answer 17

Cytosol

Question 18

Where does Oxidative decarboxylation occur?

Answer 18

Mitochondrial Matrix

Question 19

Where does the Kreb’s Cycle occur?

Answer 19

Mitochondrial Matrix

Question 20

Citrate Synthase fxn…..

Answer 20

Step 1: TCA cycle

• Oxaloactetate + Acetyl CoA—-> Citrate

Question 21

Aconitase fxn…..

Answer 21

Step 2 & 3: TCA cycle

• Citrate—–> Cis-Aconitate
• Cis-Aconitate—–> Isocitrate

Question 22

Isocitrate Dehydrogenase fxn…..

Answer 22

Step 4: TCA cycle

-Isocitrate—–> Alpha-Ketogluturate (5C) + CO2 + NADH

Question 23

Alpha-Ketoglutarate Dehydrogenase Complex fxn…

Answer 23

Step 5: TCA cycle

• Alpha-Ketoglutarate——> Suucinyl-CoA (4C) + CO2+ NADH

Question 24

Succinyl-CoA Synthase fxn…..

Answer 24

Step 6: TCA cycle

• Succinyl-CoA——> Succinate (4C) + GTP

Question 25

Succinate Dehydrogenase fxn….

Answer 25

Step 7: TCA cycle

• Succinate——> Fumarate (4C) + FADH2

Question 26

Fumarase fxn….

Answer 26

Step 8: TCA cycle

• Fumarate——-> Malate (4C)

Question 27

Malate Dehydrogenase fxn….

Answer 27

Step 9: TCA cycle

• Malate——> Oxaloacetate (4C) + NADH

Question 28

Coenzyme NAD+ contains….

Answer 28

Nicotinamide Adenine Dinucleotide

• ADP
• Ribose
• Nicotinamide (from niacin, B3)

Question 29

When does NAD+ reduce to form NADH?

Answer 29

When Nicotinamide group accepts H+ and 2e-‘s

Question 30

Coenzyme FAD contains….

Answer 30

Flavin Adenine Dinucleotide

• ADP
• Riboflavin (Vitamin B2)

Question 31

When does FAD reduce to FADH2?

Answer 31

When flavin accepts 2H+ and 2e-‘s