Lecture 17 and 18

Question 1

Define recombinant DNA.

Answer 1

Two or more DNA molecules from different origins unites into new combinations of information

Question 2

What are plasmids?

Answer 2

Small pieces of DNA that encode non-essential functions

Question 3

What do fungi produce that kill bacteria in order to gain more resources?

Answer 3

Secondary metabolites

Question 4

Why are resistance genes for metabolites stored on plasmids?

Answer 4

• Metabolites aren’t always present in the environment

- Plasmids can spread horizontally

Question 5

What are restriction enzymes?

Answer 5

Cleave DNA molecules at specific base sequences, used to identify DNA fragments

Question 6

What is the name of the plasmid for resistance in the experiment?

Answer 6

plasmid Stanley Cohen 101/102

Question 7

What does the Stanley cohen plasmid do?

Answer 7

Give resistance to terocycline

Question 8

Cohen done what?

Answer 8

Inserted DNA for tetrocycline reistance into pSC102 to see if recombinant plasmid could be made