Lecture 17 Flashcards
Compare and contrast the similarities and differences between DNA Pol I and DNA Pol III of E. coli.
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Describe how DNA is synthesized from its 5’ to 3’ end.
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Explain the significance of the origin of replication.
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Explain the problems associated with unwinding of the DNA double helix.
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Describe the role of helicase enzymes in unwinding DNA prior to its replication.
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Describe the role of single-strand binding protein & topoisomerases in DNA replication.
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Outline the order of events from RNA priming to completed DNA during replication in E.coli. (Fig 29.16 Lippincott’s)
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Distinguish between synthesis of leading and lagging strands of DNA. Identify the significance of DNA ligase in lagging strand synthesis.
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Analyze the significance of the various proteins and enzymes required for replication. Predict the effects of mutations of these proteins and enzymes on DNA replication.
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Compare and contrast the action of Type I and Type II topoisomerase enzymes
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Evaluate the proofreading activity of DNA polymerase.
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Differentiate between DNA replication in eukaryotes and prokaryotes.
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Describe the role of telomeres and telomerase in eukaryotic DNA replication.
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Outline the mechanisms of action of drugs that interfere with DNA replication.
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Clinical examples utilized in lectures:
•Inhibitors of DNA gyrase – Quinolone drugs (Ciprofloxacin)
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