Lecture 13: Brejc et al., 2001

Question 1

What are the residues involved in binding on the principal face?

Answer 1

loop A (TyrA89), loop B (TrpA143), loop C (Tyr A185, double cys A187, A188, TyrA192)

Question 2

What are the residues involved in binding on the complementary face?

Answer 2

loop D (TrpB53, GlnB55), loop E (ArgB104, ValB106, LeuB112, MetB114), loop F (TyrB164)

Question 3

What is the ordering of subunits?

Answer 3

Originally thought it was alpha-gamma-alpha-delta-beta with binding between alpha and delta and aloha and gamma

Structure is actually alpha-gamma-alpha-beta-delta