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Histopathology > Lecture > Flashcards

Lecture Flashcards

1
Q

HOW LONG SHOULD YOU LEAVE SECTIONS IN THE OVEN?

A

30 minutes

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2
Q

THIS IS AN EXAMPLE OF AN ACCENTUATOR.

A

phenol in carbon thionine

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3
Q

True of Mordants

a. insoluble
b. does not participate in the staining reaction
c. phenol in carbon fuchsin
d. does not form a complex

A

Insoluble

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4
Q

THIS IS AN EXAMPLE OF A METACHROMATIC STAIN.

A

toluidine blue

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5
Q

Examine living cells (tissues) that have been removed from an organism.

A

supravital stain

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6
Q

Demonstrate carbohydrates and other substances in the tissue.

A

Periodic Acid Schiff stain

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7
Q

What do you expect to see if you use Langhan’s Iodine stain?

A

Glycogen pink to red

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8
Q

What is the counterstain used in the best carmine method?

A

Ehrlich’s hematoxylin

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9
Q

This is useful for staining encapsulated fungi, e.g. Cryptococcus neoformans.

A

Mucicarmine Stain

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10
Q

What is the color produced if basic fuchsin is added in the mixture for carbohydrate staining?

A

bright magenta

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11
Q

best stained by silver impregnation techniques

A

reticulin fibers

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12
Q

Stain for elastic fibers

a. Verhoeffs
b. Kraijan
c. Weigerts
d. all the choices are correct

A

d. all the choices are correct

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13
Q

a rapid method for staining elastic fibers, fibrin and amyloid, employing congo red

A

kraijan

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14
Q

stain for basement membrane

A

Jones

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15
Q

Method for amyloid demonstration

a. gram’s iodine stain
b. congo red method
c. induced fluorescence staining with thioflavine
d. all the choices are correct

A

d. all the choices are correct

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16
Q

stain for lipids performed on frozen section

A

sudan black

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17
Q

a good stain to identify macrophages

A

non specific esterase

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18
Q

stain for calcium except

a. PTAH
b. Alizarin Red
c. Von Kossa
d. no exception

A

a. PTAH

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19
Q

how does reticulin fibers appear in van gieson’s stain

A

faint pinkish color

20
Q

how does reticulin fibers appear in PAS stain

A

purplish red

21
Q

collagen in van gieson’s stain

A

red

22
Q

muscle in van gieson’s stain

A

yellow

23
Q

collagen in Masson’s trichrome stain

A

blue

24
Q

collagen in Gomori’s One Step Trichrome stain

A

green

25
Q

Muscle fibers in Mallory’s Aniline Blue Stain

A

red

26
Q

What is an endogenous pigment seen in erythrocytes that is derived from Plasmodium infection?

A

Hemozoin

27
Q

Which among the following cannot detect iron in tissues?

a. Leuco patent blue V stain
b. Turnbulls blue stain
c. Rhoadanine Stain
d. Prussian blue stain

A

d. Prussian blue stain

28
Q

When stained with Gomori’s Methenamine silver stain, monosodium crystals would appear?

A

black

29
Q

The presence of asbestos bodies in lung tissue is due to deposition of?

A

silica

30
Q

Apathy’s media is used when staining for this substance:

A

copper

31
Q

Which among the following is seen in Fouchet’s solution?

A

tricholoracetic acid and ferric chloride

32
Q

This is an indirect method of identifying calcium in tissues.

A

Von-Kossa method

33
Q

What fixative is best for the demonstration of monosodium urates?

A

95% alcohol

34
Q

Which among the following will not stain myelin?

a. Holzer Stain
b. Weil’s method
c. Weiger-Pal
d. Luxol Fast Blue

A

Holzer Stain

35
Q

Which among the following will not stain Nissl bodies?

a. methylene blue
b. hematoxylin and eosin
c. toluidine blue
d. thionine
e. none of the above

A

e. none of the above

36
Q

Cajal’s Gold sublimate is used to stain?

A

astrocytes

37
Q

Which stain is not helpful for viewing neuritic plaques in Alzheimer’s disease?

A

Baker’s Chromate-Acid Hematin

38
Q

Bone marrow specimen are used for the diagnosis of the following, except

a. hemochromatosis
b. leukemias
c. fever of unknown origin
d. metastasis
e. none of the choices

A

e. none of the choices

39
Q

Most commonly employed site for BMA aspirate and biopsy collection.

A

Posterior

40
Q

A type of smear that gives an excellent detail of cell morphology

A

spread

41
Q

Simple protein

A

albumin

42
Q

Acid dyes are attracted to acidic substances.

A

False

43
Q

A fluorescent staining that gives orange red color for DNA and RNA

A

Rhodamine

44
Q

During fixation, LIPIDs are retained from tissue during processing

A

False

45
Q

Adequate solvent for Oil Red O and Sudan Black

A

70% alcohol

46
Q

When using Sudan Black B stain, nuclei will yield what color?

A

red

47
Q

NILE Blue Sulfate method, neutral lipids are dissolved by:

A

Red Oxazine

Histopathology (2 decks)

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