Lecture 10: Principles of Vesicular Traffic

Question 1

Steps of vesicular traffic:

Answer 1

1. Budding
2. Uncoating
3. Transport
4. Tethering
5. Docking
6. Fusion

Question 2

Budding is initiated by the interaction of a small ____ with the donor membrane

Answer 2

• GTP-binding protein
  
  • ARF for COPI
  • SAR for COPII

Question 4

SAR1 binding ___ coat association

Answer 4

nucleates

Question 5

SAR1 ____ leads to uncoating (molecular timer)

Answer 5

GTPase activity

Question 6

Sar1 is activated via ___

Answer 6

Sar1 GEF which removes the bound GDP, allowing GFP to bind

Question 7

COPII can adapt to larger cargo

Answer 7

Question 8

What are the advantages of transporting along cytoskeletal tracks?

Answer 8

These tracks limit the number of possible destinations and aid in proper targeting

Question 9

Tethering vs Docking

Answer 9

The primary difference between tethering and docking is the physical distance between the vesicle membrane and the target membrane

Question 10

The operational definition of docking is the interaction of ____ proteins

Answer 10

SNARE

Question 11

____ proteins can be used to identify different compartments

Answer 11

SNARE

Question 12

____ proteins facilitate transport vesicle docking

Answer 12

Rab

Question 13

Activated ____ can organize specialized membrane patches that actively recruit proteins to a fusion site

Answer 13

Rabs

Question 14

Rabs proteins cooperate as organelles mature

Answer 14

Question 15

SNARE _____ drives membrane fusion

Answer 15

pairing

Question 16

How do SNAREs drive membrane fusion?

Answer 16

v-SNAREs and t-SNAREs interact to form a stalk, pulling the two membranes closer until the meet and fuse together

Question 17

What are responsible for recycling and unpairing cis-SNARE complexes?

Answer 17

NSF and SNAPs