Lecture 10 Flashcards

1
Q

How to make a genomic library IN ORDER

A

1) DNA purification
2) Restriction enzyme
3) Plasmid
4) Transformation
5) Selection

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2
Q

How to make a cDNA library IN ORDER

A

1) mRNA purification
2) poly(T)primer
3) RT-PCR
4) Restriction enzyme
5) Clone into plasmid
6) Transformation

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