Lecture 10

Question 1

Which factors lead to growth in quiescent cells?

Answer 1

E2F, myc, cyclin E

Question 2

Which 2 genes does E2F activate that lead to p53 activation?

Answer 2

ATM and ARF

Question 3

What are the 4 p53 pro-apoptotic cofactors?

Answer 3

JMY, ASPP1, SPP2, DINP1

Question 4

What does p53 activate that leads to growth arrest?

Answer 4

P21

Question 5

Myc works as a heterodimer with

Answer 5

Max

Question 6

When Max binds mad, what’s the effect?

Answer 6

Repression of mitogenic signals, growth inhibition

Question 7

What is MAD?

Answer 7

a repressor of myc - binds max instead of myc,

Question 8

c-myc

Answer 8

overexpression in many tumors, translocation in burkytt’s lymphoma

Question 9

n-myc

Answer 9

amplification in neuroblastoma

Question 10

l-myc

Answer 10

amplification and rearrangement in lung cancer

Question 11

Can myc alone transform cells? Why?

Answer 11

No, because of the fail safe mechanism and bc myc and many myc targets require post translational modifications

Question 12

What does overexpression of beta catenin cause?

Answer 12

Cancer

Question 13

What are beta-catenin’s jobs in the cell?

Answer 13

Cell adhesion and transcription regulation

Question 14

What does Beta Catenin activate when it goes tot he nucleus?

Answer 14

Cyclin D and myc, which cause proliferation

Question 15

What, other than proliferation, can beta-catenin activate?

Answer 15

It can transcriptionally activate Arf –> activate p53 –> apoptosis