Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Renal I Final > Lecture 1: Renal Transport Mechanisms > Flashcards

Lecture 1: Renal Transport Mechanisms Flashcards

1
Q

5 distinct barriers for substance to be reabsorbed?

A

1) Leave tubular fluid by crossing luminal membrane
2) Through cytosol of tubular cell
3) Cross basolateral membrane of tubular cell to enter interstitial fluid
4) Diffuse through interstitial fluid
5) Penetrate the capillary wall to enter plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Why bother to filter 180L/day and then reabsorb 99% of it?

A
  1. Foreign substances are filtered into the tubule, but NOT reabsorbed into the blood
  2. Filtering ions and water into the tubule make regulation simple
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Majority of substances are reabsorbed where; what are they?

A

Proximal convoluted tubule

100% of glucose, AA’s
70% of Water, Na+, K+ Phosphate, and Ca2+
50% Urea

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What is reabsorbed in the proximal straight tubule?

A

Phosphate (15%)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Primary vs Secondary active transport?

A

Primary: coupled directly to an energry source (ATP)

Secondary: coupled indirectly to an energy source (due to concentration gradient of an ion)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Primary active transporters of the kidneys?

A

Na-K+ ATPase, Hydrogen ATPase, Hydrogen-K+ ATPase, and Calcium ATPase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Key element of proximal tubule reabsorption; location?

A

Na-K+ ATPase; basolateral membrane

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is tubular load and the calculation?

A

Amount of any substance(s) entering the tubule/min

TL(s) = P(s) x GFR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What 2 things does the operation of Na+-K+ ATPase accomplish?

A

1) Lowers IC [Na+] and increases IC [K+]

2) Creates low IC [Na+] and negative charge

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Describe the Transcellular vs Paracellular route

A

Transcellular: substances move through the luminal membrane, cytosol, and the basolateral membrane

Paracellular: substances move between the tubule cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Why is movement through the paracellular route limited?

A

Contain tight junctions, which are leaky and only allow some important ions (Ca2+, Mg2+, K+ and some Na+)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

How do substances move through transcellular vs paracellular (transport mechanism)?

A

Transcellular: Passive diffusion or Active transport

Paracellular: Diffusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the single most abundant cation in filtrate; how much energy is devoted to their reabsorption; almost always via what mechanism and route?

A

Na+; 80%; almost always active transport and via the transcellular route

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Majority of Na+ ion reabsorption occurs through what channels on what surface?

A

Sodium leak channels on the apical surface (luminal membrane) of the proximal tubule cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Sodium, H2O, and other substances are reabsorbed from the interstitial fluid into the peritubular capillaries by which forces?

A

Hydrostatic (Pi) and colloid osmotic pressure gradients (πc)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

How does the diffusion of Na+ out of the cell using Na-K+ ATPase favor the movement of more Na+ into the cell?

A

The Na-K+ ATPase is working to get Na+ out of cell across the basolateral membrane, creating a low intracellular [Na+] and a negative intracellular electrical potential. This negative electrical potential in the cell attracts more Na+ from the tubular lumen to diffuse into the cell so that it can then be pumped out and into the interstitium for reabsorption into the capillary.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

80% of the Na+ entering the tubule cells does so in exchange for what ion, via what transporter?

A

Exchange for H+ (secretion) via the Na-H+-exchanger 3 (NHE3) - Countertransporter/Antiporter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the exchange ratio for the NHE3 antiporter?

A

One Na+ is reclaimed from the lumen in exchange for one H+ ion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

When H+ gets exchanged for Na+ by NHE3 you now have an acidic H+ ion in the lumen of the proximal tubule, what happens?

A
  • H+ secreted into the filtrate combines with HCO3 —> H2CO3, which is quickly converted to H2O and CO2 via carbonic anhydrase
  • CO2 diffuses back into the tubule cell cytosol where it combines with H2O to form H2CO3 in the presence of carbonic anhydrase. H2CO3 then dissociates into H+ and HCO3.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Once H2CO3 has dissociated in the tubular cell cytosol to form H+ and HCO3, what occurs?

A

H+ recycles through the NHE3 again, while HCO3 moves into the interstitial space via the NaHCO3 cotransporter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

The NHE3 transporter is important for the reabsorption of what ions?

A

Na+, Cl-, and HCO3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Discuss chloride reabsoprtion

A

More H20 than Cl- is reabsorbed in the first 1/2 of the proximal tubule, which causes the [Cl-] in tubular fluid to increase about 20% along the length of the proximal tubule. This increasing concentration provides a chemical gradient that drives Cl- movement “passively” w/o need for additional energy expenditure, along the PARACELLULAR pathway.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What is present in the cell membranes of the thin descending limb, thick ascending limb, and collecting duct that determines the movement of H2O?

A

Thin descending: smaller amount of tight junctions, so paracellular movement of H2O occurs freely

Thick ascending/collecting duct: abundant tight junctions prohibit water movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What prevents the transcellular movement of water?

A

Specialized epithelial cells in the apical membrane (luminal membrane), which are tightly packed with lipids, repelling or blocking water movement through the lipid bilayer.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

What is the large class of proteins that allow the transport of water via the transcellular route?

A

Aquaporins

26
Q

Where is Aquaporin I located?

A

Membranes of the cells lining the proximal tubule and thin descending limb

27
Q

Where is Aquaporin II found?

A

Apical membrane of the collecting duct cells

28
Q

Where is Aquaporin III and IV found?

A

Basolateral membrane of the collecting duct

29
Q

What makes Aquaporin II unique?

A

Subject to control mechanisms, responding to the needs of the body for water. Regulated by ADH.

30
Q

Glucose is almost completely reabsorbed from the tubules via which mechanism?

A

Active transport molecules called SGLT’s

31
Q

What is SGLT1?

A

High-affinity, low capacity transport proteins, responsible for 10% of glucose reabsorption and are found in the distal part of the PCT.

*High-affinity means they are active even with low concentrations of filtrate glucose

32
Q

What is SGLT2?

A

Low-affinity, high capacity transport proteins, responsible for 90% of reabsorption, found in the first part of PCT (S1 and S2)

*Low affinity means it requires a higher concentration to fill its binding sites

33
Q

What do SGLT’s link with to help them move glucose?

A

Link with the movement of Na+ down its electrochemical gradient to the “uphill” reabsorption of glucose

34
Q

How does glucose move into the interstitial space once inside the cell; which transporters for each part of the PCT?

A
  • GLUT2, in the S1 segment of the PCT

- GLUT 1 in the S3 segment of the PCT

35
Q

Describe the overall movement of glucose from inside PCT to the cell and then to the interstitium.

A

Glucose (S1, S2)–> SGLT 2 –> Cell —> GLUT2–> Interstitium

Glucose (S3)–> SGLT 1–> Cell–> GLUT1 –> Interstitium

36
Q

Since glucose is freely filtered across the glomerulus what is the concentration within the tubule vs. the interstitium and blood?

A

The same

37
Q

What is one of the pharmacological interventions for Type 2 DM?

A

SGLT2 inhibition, will cause less glucose to be reabsorbed and more to be excreting, decreasing the concentrations in the blood

38
Q

What is the transport maximum for glucose (Tm); plasma glucose level of; what occurs past Tm?

A
  • SGLT1 and SGLT2 will absorb filtrate glucose until all of their receptor sites are full, this is the Tm.
  • Corresponds to a plasma glucose level of about 200 mg/dL.
  • At this point extra glucose will pass into the bladder and will be excreted (glycosuria)
39
Q

What is permeable and impermeable in the descending loop of Henle; creates what osmolarity?

A
  • H20 is permeable (25% reabsorbed)
  • Solutes NaCl impermeable
  • Fluid becomes hyper-osmolar
40
Q

How does reabsoprtion work in the thick ascending limb of the loop of Henle?

A
  • Na+ and Cl- can be reabsorbed, while H20 is impermeable.

- The filtrate becomes very dilute and hypo-osmolar by the time it gets to the distal tubule

41
Q

How is the thick ascending loop able to accomplish the task of intense particle reclamation?

A

Has very high density of Na/K ATPase along its basolateral membrane

42
Q

Function of the Na-K-2Cl co-transporter; found where?

A
  • Found on apical membrane of thick ascending limb
  • Allows one Na+, one K+, and two Cl- ions to cross the apical membrane. K+ subsequently exits the cell via an apical channel back into tubular fluid, while the Cl- exits the basolateral side of the cell via a chloride channel, and the Na+ exits via Na/K ATPase.
43
Q

What is the net effect of the Na-K-2Cl co-transporter mechanism?

A

One positive charge and two negatively charged particles have been reabsorbed from the lumen; leading to a transepithelial positive voltage.

44
Q

Importance of the transepithelial positive voltage created by the Na-K-2Cl co-transporter; movement is via what kind of reabsorption pathway?

A
  • Drives the movement of cations, Na+, Ca2+, and Mg2+ from the lumen to the basolateral side via paracellular reabsoprtion.
  • Helps regulate the serum levels of Ca2+ and Mg2+, two critical cations
45
Q

How is the majority of K+ reabsorbed in the PCT?

A

60% is reabsorbed actively by the paracellular transport mechanism

46
Q

What establishes a concentration gradient for K+ reabsorption in the paracellular space?

A

Active K+ uptake via Na+/K+ ATPase located in the lateral cell membrane facing the lateral intercellular spaces

47
Q

K+ that enters the cell actively from lateral surface is able to exit the basolateral membrane via which 3 pathways?

A

1) The conductive K+ channel
2) The K+/Cl- co-transporter
3) The Na+/K+ ATPase pump

48
Q

What do high K+ diets lead to in regards to K+ channels?

A

Insertion of apical channels and therefore higher K+ secretion

49
Q

Hyperkalemia from either high K+ diet or other factors causes what?

A

Stimulates K+ secretion within minutes

50
Q

Hypokalemia from either low K+ diet or other factors causes what?

A

Decreases K+ secretion

51
Q

What is the major mechanism of transport for NaCl in the distal tubule?

A

Na+-Cl- cotransport (symporter)

52
Q

What is a major site for pharmacological intervention in the distal tubule; how do they work?

A

The Na+-Cl- symporter can be blocked by Thiazide diuretics

They act to increase the excretion of Na+ and Cl- by inhibiting the Na+-Cl- symporter.

53
Q

How do Thiazides affect Ca2+?

A
  • Reduce the urinary excretion of Ca2+, enhancing reabsorption in the DCT.
  • Are used to treat kidney stones and may be useful for treating osteoporosis
54
Q

What causes the release of aldosterone from the adrenal cortex?

A

Angiotensin II or increased plasma K+

55
Q

Which 3 parts of the kidney does aldosterone have an effect on?

A

1) Principal cells of the collecting ducts,
2) Distal portion of DCT
3) Thick ascending loop

56
Q

What does a rise in plasma K+ cause aldosterone to do?

A

Stimulates the release of aldosterone, which in turn increases the tubular secretion and urinary excretion of K+. At the same time it increases the tubular reabsorption of Na+ and decreases urinary excretion of Na+

57
Q

Which two pathways can cause aldosterone secretion?

A

1) A rise in plasma K+

2) Fall in plasma Na+ stimulates RAAS pathway

58
Q

Calcium homeostasis is regulated by what 3 hormones?

A

1) PTH
2) Calcitonin
3) 1a,25-dihydroxy-Vitamin D

59
Q

3 major effects of PTH

A

1) Directly stimulates bone resorption
2) Directly stimulates recovery of calcium in the kidney
3) Stimulates the production of 1a,25-dihydroxy-Vitamin D from its precursors

60
Q

What has the opposite effects of PTH in bone and kidney?

A

Calcitonin

61
Q

Key to calcium homeostasis is?

A

Amount of calcium absorbed in the gut, because there are always some losses of calcium in the feces, urine, and sweat

Renal I Final (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions