LECTURE 1 : PIPETTING Flashcards
learn pipetting to know how to cling (?) HAKDOG lol k bye
A tool used for accurately measuring and transferring small volumes of liquid.
PIPETTES
- 1-1000 uL
- used in Molbio Lab
MICROPIPETTES
- > 1000 uL
MACROPIPETTES
CLASSIFICATION OF PIPETTE BASED ON DESIGN:
- Filled to particular graduation & delivers less the volume
TC / TO CONTAIN PIPETTE
CLASSIFICATION OF PIPETTE BASED ON DESIGN:
- Take slightly more than actual volume, but deliver the actual amount.
TO DELIVER PIPETTE
CLASSIFICATION OF PIPETTE BASED ON DRAINAGE:
- With etched rings
- must be blowout (using rubber bulb)
BLOWOUT
TYPES OF PIPETTE:
- Have graduated scale marked along the length of the pipette allowing precise measurement of different volume.
Ex. Serologic, Mohr, Bacteriologic, Kolmer, Micropipette
MEASURING/GRADUATED PIPETTE
CLASSIFICATION OF PIPETTE BASED ON DRAINAGE:
- without etched rings
- drained by gravity
SELF-DRAINING
TYPES OF MICROPIPETTES BASEND ON WORKING PRINCIPLE:
- Consist of PISTON which aspirates & dispenses liquid samples as the air pocket moves up & down.
AIR DISPLACEMENT MICROPIPETTE
TYPES OF PIPETTE:
- used for transferring liquids from one container to another,
- do not typically have graduations for precise measurement.
Ex, Volumetric, Ostwald, Pasteur, Micropipette
TRANSFER PIPETTE
TYPES OF MICROPIPETTES BASED ON WORKING PRINCIPLE:
- Piston comes in DIRECT CONTACT of the sample
- MICROSYRINGE composed of a CAPILLARY
- For PROBLEM SAMPLES
POSITIVE DISPLACEMENT MICROPIPETTE
What is the recommended pipette for AQUEOUS SAMPLES
AIR DISPLACEMENT PIPETTES
TYPES OF MICROPIPETTES BASED ON OPERATING MECHANISM:
- Automated
- Aspirating & dispensing of liquid is performed by the one-touch buttons instead of manual plunger.
ELECTRONIC MICROPIPETTE
What is the recommended pipette for CORROSIVE SAMPLES?
POSITIVE DISPLACEMENT PIPETTES
What is the recommended pipette for BIOLOGICAL SAMPLES
AIR DISPLACEMENT WITH FILTER TIPS
TYPES OF MICROPIPETTES BASED ON OPERATING MECHANISM:
- Operated manually based on a piston-shaft spring mechanism
MECHANICAL MICROPIPETTE
TYPES OF MICROPIPETTES BASED ON NUMBER OF CHANNELS
- Has single channel to aspirate
SINGLE CHANNEL MICROPIPETTE
TYPES OF MICROPIPETTES BASED ON NUMBER OF CHANNELS
- Has multiple channels to aspirate
- Variants: 8, 12, 16 channel
MULTICHANNEL MICROPIPETTE
TYPES OF MICROPIPETTES BASED ON VOLUME/CAPACITY
- Volume of liquid remains fixed
- Used when the same volume of liquid is to be dispensed multiple times.
FIXED VOLUME MICROPIPETTE
TYPES OF MICROPIPETTES BASED ON VOLUME/CAPACITY
- Has a specific minimum & maximum volume range
- can be adjusted depending upon the requirement of the user.
VARIABLE VOLUME MICROPIPETTE
SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with all single channel pipettes foe sample preparation
Test tube & Centrifuge tubes
MICROPIPETTE STRUCTURE
PLUNGER
TIP EJECTOR
VOLUMES WINDOW/VOLUMETRIC DISPLAY
SHAFT
DISPOSABLE
SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with POSITIVE-DISPLACEMENT PIPETTES and pipette fillers with plastic.
LONG TEST TUBES
SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Ideal for dispensing reagents especially with multichannel
REAGENT RESERVOIRS
SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with AIR DISPLACEMENT
96-WELL & 384-WELL MICROPIPETTES AS WELL AS 8-STRIPS
WHEN TO CHANGE A TIP?
- When transferring single samples of different
liquids - For repetitive dispensing of the same liquid.
