LECTURE 1 : PIPETTING

Question 1

A tool used for accurately measuring and transferring small volumes of liquid.

Answer 1

PIPETTES

Question 2

• 1-1000 uL
• used in Molbio Lab

Answer 2

MICROPIPETTES

Question 3

• > 1000 uL

Answer 3

MACROPIPETTES

Question 3

CLASSIFICATION OF PIPETTE BASED ON DESIGN:
- Filled to particular graduation & delivers less the volume

Answer 3

TC / TO CONTAIN PIPETTE

Question 3

CLASSIFICATION OF PIPETTE BASED ON DESIGN:
- Take slightly more than actual volume, but deliver the actual amount.

Answer 3

TO DELIVER PIPETTE

Question 4

CLASSIFICATION OF PIPETTE BASED ON DRAINAGE:
- With etched rings
- must be blowout (using rubber bulb)

Answer 4

BLOWOUT

Question 5

TYPES OF PIPETTE:
- Have graduated scale marked along the length of the pipette allowing precise measurement of different volume.
Ex. Serologic, Mohr, Bacteriologic, Kolmer, Micropipette

Answer 5

MEASURING/GRADUATED PIPETTE

Question 5

CLASSIFICATION OF PIPETTE BASED ON DRAINAGE:
- without etched rings
- drained by gravity

Answer 5

SELF-DRAINING

Question 6

TYPES OF MICROPIPETTES BASEND ON WORKING PRINCIPLE:
- Consist of PISTON which aspirates & dispenses liquid samples as the air pocket moves up & down.

Answer 6

AIR DISPLACEMENT MICROPIPETTE

Question 6

TYPES OF PIPETTE:
- used for transferring liquids from one container to another,
- do not typically have graduations for precise measurement.
Ex, Volumetric, Ostwald, Pasteur, Micropipette

Answer 6

TRANSFER PIPETTE

Question 7

TYPES OF MICROPIPETTES BASED ON WORKING PRINCIPLE:
- Piston comes in DIRECT CONTACT of the sample
- MICROSYRINGE composed of a CAPILLARY
- For PROBLEM SAMPLES

Answer 7

POSITIVE DISPLACEMENT MICROPIPETTE

Question 8

What is the recommended pipette for AQUEOUS SAMPLES

Answer 8

AIR DISPLACEMENT PIPETTES

Question 9

TYPES OF MICROPIPETTES BASED ON OPERATING MECHANISM:
- Automated
- Aspirating & dispensing of liquid is performed by the one-touch buttons instead of manual plunger.

Answer 9

ELECTRONIC MICROPIPETTE

Question 9

What is the recommended pipette for CORROSIVE SAMPLES?

Answer 9

POSITIVE DISPLACEMENT PIPETTES

Question 9

What is the recommended pipette for BIOLOGICAL SAMPLES

Answer 9

AIR DISPLACEMENT WITH FILTER TIPS

Question 10

TYPES OF MICROPIPETTES BASED ON OPERATING MECHANISM:
- Operated manually based on a piston-shaft spring mechanism

Answer 10

MECHANICAL MICROPIPETTE

Question 11

TYPES OF MICROPIPETTES BASED ON NUMBER OF CHANNELS
- Has single channel to aspirate

Answer 11

SINGLE CHANNEL MICROPIPETTE

Question 11

TYPES OF MICROPIPETTES BASED ON NUMBER OF CHANNELS
- Has multiple channels to aspirate
- Variants: 8, 12, 16 channel

Answer 11

MULTICHANNEL MICROPIPETTE

Question 11

TYPES OF MICROPIPETTES BASED ON VOLUME/CAPACITY
- Volume of liquid remains fixed
- Used when the same volume of liquid is to be dispensed multiple times.

Answer 11

FIXED VOLUME MICROPIPETTE

Question 12

TYPES OF MICROPIPETTES BASED ON VOLUME/CAPACITY
- Has a specific minimum & maximum volume range
- can be adjusted depending upon the requirement of the user.

Answer 12

VARIABLE VOLUME MICROPIPETTE

Question 13

SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with all single channel pipettes foe sample preparation

Answer 13

Test tube & Centrifuge tubes

Question 14

MICROPIPETTE STRUCTURE

Answer 14

PLUNGER
TIP EJECTOR
VOLUMES WINDOW/VOLUMETRIC DISPLAY
SHAFT
DISPOSABLE

Question 15

SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with POSITIVE-DISPLACEMENT PIPETTES and pipette fillers with plastic.

Answer 15

LONG TEST TUBES

Question 15

SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Ideal for dispensing reagents especially with multichannel

Answer 15

REAGENT RESERVOIRS

Question 15

SPECIFIC MICROPIPETTES FOR SPECIFIC VESSELS:
- Used with AIR DISPLACEMENT

Answer 15

96-WELL & 384-WELL MICROPIPETTES AS WELL AS 8-STRIPS

Question 16

WHEN TO CHANGE A TIP?

Answer 16

• When transferring single samples of different
  liquids
• For repetitive dispensing of the same liquid.