Lecture 1

Question 1

What is a pluripotent cell

Answer 1

A cell with the ability to differentiate into many different cells from different organ systems

Question 2

What is a Progenitor cell?

Answer 2

A cell that is either uni or multipotent and can only give rise to a very limited number of differentiated cells, e.g. RBC precursores

Question 3

Somatic Cell Transfer

Answer 3

When the nucleus of a cell is isolated and implanted into another cell which lacks a nucleus

Question 4

What is flow cytometry

Answer 4

seperation of cell types based on the amount of light scatter

Question 5

What is a primary culture?

Answer 5

A laboratory culture of cells generated from cells taken from a living organism and separated with the use of chelating agents

Question 6

What is a Cell strain?

Answer 6

Cell lineage derived from a primary culture

Question 7

What are the three patterns of stem cell division?

Answer 7

Maintain stem cell population, increase stem cell population, decrease stem cell population

Question 8

What are transient amplifiers?

Answer 8

Progentior cells that divide rpaidly

Question 9

What are the advantages of using yeast?

Answer 9

Simple and cheap to culture, Availability of gene knockouts for the entire genome

Question 10

What is a totipotent cell?

Answer 10

A cell that can proliferate into any type of cell

Question 11

What would you use yeast to study?

Answer 11

Control of cell cycle and division, Protein synthesis and secretion, function of the cytoskeleton, cell differentiaition, aging, gene regulation and chromosoe structure

Question 12

What are the advantages of using round worm?

Answer 12

Cell lineage of all adult cells are know, knock out of single genes are avaliable

Question 13

What would you use round worm to study?

Answer 13

development of the body plan and the nervous system, cell lineage and programmed cell death, cell proliferation, cancer genes, aging, behavior, gene regulation and chromosome structure

Question 14

What would you use the fruitfly to study?

Answer 14

Development of body plan, nervous system, heart and musculature, generation of cell lineage and pogrammed cell death, genetic control of behaviour, cancer gneers and control of cell proliferation, affect of cell polarisation and physiologically active chemicals

Question 15

What are the advantages of using a zebrafish?

Answer 15

Transparent eggs, have a simple backbone

Question 16

What would you use a zebrafish to study?

Answer 16

Birth defects, cancer, formation and function of brain and nervous system, development of vertebrae tissues

Question 17

What are the advantages of using a mouse?

Answer 17

Simple gene knock ins and outs dues to embryonic stem cells

Question 18

What would you use a mouse to study?

Answer 18

Development of body tissues, brain and nervous system, immune system function, models for human diseases, infectious diseases, gene regulation and inheritance