Lecture 1

Question 1

HCAII is an enzyme limited by what?

Answer 1

Diffusion of the substrate to its active sites

=

Diffusion limited enzyme

Question 2

Kc of HCAII?

Answer 2

?

Question 3

List of roles for HCAII

Answer 3

?

Question 4

HCAII role in plants

Answer 4

Bicarbonate to co2 for rubisco enzyme for energy creation in Calvin cycle

Question 5

How does HCAII function for ion transport?

Answer 5

Membrane bound HCAII uses law of mass action to force the flow of certain ions by converting only on one side of membrane

Question 6

Disease relevant to HCAII?

Answer 6

Glaucoma
= too much fluid pressure
= fluid flows bc of HCAII

Question 7

Why don’t soda or bread need enzyme for bicarbonate to co2 reaction?

Answer 7

Driven by pressure and heat respectively

Question 8

Describe HCAII structure

Answer 8

Alpha beta protein

More to come

Question 9

Is HCAII an example of convergent evolution?

Answer 9

Yes

Question 10

How many isoforms of human HCAII are there?

Answer 10

7

Question 11

Isoform definition

Answer 11

?

Question 12

What type of enzyme is HCAII?

Answer 12

Metalloenzyme

Question 13

What does zinc act as within HCAII?

Answer 13

Lewis acid
Engages nucleophile

Question 14

What is the nucleophile that zinc engages?

Answer 14

Hydroxyl group => attacks co2 to form carbonic acid

Question 15

Write out steps for enzyme rxn on flash card

Question 16

RLS for HCAII

Answer 16

Step #6
Base coming in for proton