Lecture 1

Question 1

Automated culture system

Answer 1

BACTEC FX

Question 2

MALDI-TOF

Answer 2

Laser beam to vaporize à forms cloud of + charge –>electrostatic field

Question 3

MRSA PCR Nasal Test

Answer 3

Nasal swab to identify presence/absence of MRSA in nares that serve as surrogate marker for respiratory track–> if (-), 95% change we can
rule out MRSA

Question 4

BioFire FilmArray Panel

Answer 4

When?–>respiratory, blood culture, GI, meningitis/encephalitis,
pneumonia, joint infection

What?–>PCR to detect variety of pre-determined pathogens
associated with infection

Question 5

Verigene Panel

Answer 5

When?–>respiratory, blood culture, GI

What?–>PCR to detect variety of pre-determined pathogens
associated with infection

Question 6

PhenoTest BC Kit–>FISH

Answer 6

Bloodstream infection

Susceptibility
Organism ID
Genotype

Question 7

BioFire BCID2–>PCR

Answer 7

Organism ID
Genotype

Question 8

ePLEX BCID–>PCR

Answer 8

Organism ID
Genotype

Question 9

T2 Bacteria–>PCR

Answer 9

Organism ID
Genotype

Question 10

Verigene–>PCR

Answer 10

Organism ID
Genotype

Question 11

MIC testing

Answer 11

Gold standard

Standardized bacterial inoculum added to each tube of doubling serial dilutions of antimicrobials –>ncubated for 16-24 hrs–>examined