Lecture 1 Flashcards
True or False: New cells are generated on their own.
New cells are generated only from preexisting cells.
What are the types of prokaryotic cells?
Bacteria & Archaea
What are examples of Eukaryotes?
Plants, Animals, Fungi, Protists and other complex unicellular organisms
Do prokaryotic cells have a nucleus?
No
What does the envelope surrounding prokaryotic cells consist of?
Inner plasma membrane, peptidoglycan cell wall, lipopolysaccharide outer membrane (sometimes)
Describe the structural complexity of Eukaryotic cells.
Internal membranes that organize and create intracellular compartments with different functions
Key differences between Eukaryotic and Prokaryotic cells
No cell wall in the animal cell, Chloroplast only in the plant cell
Function of plasma membrane
A selective barrier that separates a cell from its surroundings. Facilitates intra- and extracellular communication through budding and fusing of vesicles in the processes of endo- and exocytosis.
What is the plasma membrane composed of?
a phospholipid bilayer embedded with proteins and may be covered in carbohydrates.
Relationship between nucleus and DNA
Contains most of the DNA of the cell
DNA and protein combine to form chromatin
Function of mitochondria
Harness energy from food molecules to produce usable energy for the cell (ATP)
What are the three organelles enclosed within 2 membranes?
Nucleus, mitochondria, chloroplast
Difference between rough ER and smooth ER
Rough ER: studded with ribosomes which translate RNA into protein
Smooth ER: no ribosomes, involved in the synthesis / storage of lipids
Function of endoplasmic reticulum
Involved in the production of many cell components
What does the Golgi apparatus do?
Modifies and packages molecules made in the ER that are to be secreted or transported to another cell compartment
Is the cytosol contained within intracellular membranes?
no
What does the cytoskeleton do?
Govern internal organization, strength, shape, and movement
What are the requirements for light microscopy?
- Focus light through a condenser
- Specimen prepared properly (ex. Fixed or not?)
Lenses (objective, tube, eyepiece)
- Specimen prepared properly (ex. Fixed or not?)
Requirements for fluorescent microscopy
Similar requirements to a light microscope except:
1. Different wavelengths are filtered to the specimen (ex. Blue light is permitted to pass to the specimen in this example)
2. The 2nd filter blocks other light - only the light emitted from the sample is passed to the eyepiece (ex. Green light in this example)
transmission electron microscope
uses beams of electrons instead of beams of light yielding better resolution of tiny structures (1nm) - within the cell
Confocal fluorescent microscope
can generate an optical section of a sample to generate a 3D image
scanning electron microscope
uses electrons to image small structures - scanning the surface
central dogma
DNA encodes for genes that can transcribe to make RNA, which makes proteins - breaking things down
Endosymbiont Theory
The archaea cell ate the bacterial cell, the mitochondria became the endosymbiont, became multicellular