LECT 2 Qs Flashcards
Describe the 3 main points of One Step Growth Experiment
- Single cycle infection
- Infect every cell in population
- Achieve synchrony for infected cell population
What is m.o.i?
What is the equation?
The ratio of virus particles to cells
Total pfu’s/Total number of cells
What is the Poisson equation?
Helps determine the relationship between m.o.i and fraction of infected cells
How do you extract extracellular viruses?
Centrifuge!
How do you extract extracellular and intracellular?
Disrupt cells to do so
What is the latent phase?
The period from the beginning of infection until you detect the virus again
What is the eclipse phase?
The period from when the virus “disappears” and is detected again
When does Genome replication begin?
At the “late” phase
What is happening during the Early Phase?
Gene expression is directed toward genome replication
What is happening during the Late Phase?
Gene expression is directed toward making new viruses particles
What is burst size?
Virus yield/Total number of affected cells
What two symmetries do Naked Capsid Viruses and Enveloped Viruses have?
Helical and Cubic
What are the types of DNA genomes?
Single stranded or double stranded
What DNA shapes are there?
Linear and circular
What are the types of RNA genomes?
Single stranded or double stranded
