Lec 4

Question 1

Samples were taken from the body of the patient

Answer 1

Clinical specimen

Question 2

Attributed to disease state or infection or condition of a certain patient

Answer 2

Clinical

Question 3

Anything was taken out from the patient (blood, body fluid) that can be sent to the laboratory for examination

Answer 3

Specimen

Question 4

Collection of the specimen, laboratory receiving the specimen, instruction from the lab technologist on how the patient should collect the sample

Answer 4

Preanalytical phase

Question 5

Thick peptidoglycan layer with teichoic acid

Answer 5

Gram-positive

Question 6

Thin peptidoglycan layer with lipopolysaccharides

Answer 6

Gram-negative

Question 7

Direct observation of microorganisms under the microscope

Answer 7

Microscopic examination

Question 8

Patient detail in the frosted end

Answer 8

Name
Birthdate
Sex
Date and time of collection
Specimen source

Question 9

Thick liquids

Answer 9

Sputum/phlegm

Question 10

Semisolid

Answer 10

Feces/stool

Question 11

Best way for preparing thin smears

Answer 11

Cytocentrifugation

Question 12

Spinning the cells to concentrate and sediment

Answer 12

Centrifuge

Question 13

Aqueous solution composed of a single dye

Answer 13

Simple stain

Question 14

Not used in staining microorganisms because the morphology of the microorganisms can never be established

Answer 14

Simple stain

Question 15

Chemical of the simple stain is known as?

Answer 15

Mordant

Question 16

Simple stain

Answer 16

1. Methylene blue
2. Crystal violet
3. Carbolfuchsin
4. Safranin

Question 17

Common staining technique used in the microbiology laboratory

Answer 17

Differential stain

Question 18

Example of differential stain

Answer 18

Gram stain and Acid Fast stain

Question 19

Cheapest, most inexpensive, cost-effective procedure in the microbiology laboratory

Answer 19

Special stain

Question 20

Primary stain

Answer 20

Crystal violet

Question 21

Chemical name of Crystal violet

Answer 21

Hexamethyl-para-rosaniline chloride

Question 22

Alternative of Crystal violet if not available in the lab

Answer 22

Gentian violet

Question 23

Name of the reagent in mordant

Answer 23

Gram iodine

Question 24

Color of the Gram iodine

Answer 24

Brown colored reagent

Question 25

decolorizer reagent in differentiation/decolorization

Answer 25

Acetone alcohol

Question 26

Counterstaining

Answer 26

Safranin

Question 27

Color of the safranin

Answer 27

Red-colored stain

Question 28

Color of the acid fast organism

Answer 28

Red

Question 29

Color of the non-acid fast organism

Answer 29

Blue

Question 30

Heating or steaming the smear

Answer 30

Ziehl-Neelsen method

Question 31

Most commonly used procedure in the lab

Answer 31

Ziehl-Neelsen method

Question 32

Does not involve heat or steam the smear

Answer 32

Kinyoun staining method

Question 33

It increases the concentration of the phenols so that the acid-fast organism to be stained properly

Answer 33

Kinyoun staining method

Question 34

Cell wall of acid-fast organism is made up of?

Answer 34

Mycolic acid/Hydroymethoxyl acid