Lec 3: Methods Flashcards
methods in virology are used also in
cell & molecular biology
most cells taken from body…
don’t grow well in culture
cell line =
If cells from a primary culture can be subcultured they are growing as a cell line
continuous cell line =
cells can be subcultured only a finite number of times unless they are immortalized, which can be subcultured indefinitely as a continuous cell line
cancer cells are already…
&…
immortalized,
…& continuous cell lines may be established from these without further treatment
Normal Cells from Animal –> _____ –> —-(___)—-> ____ —-(______)—-> _____
Normal Cells from Animal –> 1* Cell Culture –> —-(subculture)—-> Cell Line —-(immortalization)—-> Continuous Cell Line
Cancer Cells from Animal –> _____
Cancer Cells from Animal –> Continuous Cell Line
primary culture cells have
finite life span
Primary culture contains
a very heterogeneous population of cells
Subculturing of primary cells leads to
the generation of cell lines
Cell lines have
limited life span, they passage several times before they become senescent
Cells such as macrophages and neurons do not
divide in vitro so can not be used as primary cultures
cell strain
Lineage of cells origination from the primary culture is called a cell strain
Continuous Cell Lines –>
HeLa cells
senescence =
Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite.
