LEC 2ND PPT BUT STAINS ONLY ETC Flashcards

1
Q

INCLUSIONS

Much Granules

A

Mycobacterium tuberculosis

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2
Q

INCLUSIONS

Babes Ernst Bodies

A

Corynebacterium diphtheriae

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3
Q

INCLUSIONS

Metachromatic Granules

A

Corynebacterium diphtheriae

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4
Q

INCLUSIONS

Volutin

A

Corynebacterium diphtheriae

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5
Q

INCLUSIONS

Halberstaedter Prowazek Bodies

A

Chlamydia trachomatis

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6
Q

INCLUSIONS

Levinthal Cole Lillie Bodies

A

Chlamydophila psitaci

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7
Q

SPECIAL STAINS

Dyar Stain

A

Cell Wall

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8
Q

SPECIAL STAINS

Albert

A

Metachromatic Granules

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9
Q

SPECIAL STAINS

Neisser

A

Metachromatic Granules

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10
Q

SPECIAL STAINS

Fulton-Schaeffer

A

Endospores

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11
Q

SPECIAL STAINS

Dorner

A

Endospores

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12
Q

SPECIAL STAINS

Gray

A

Flagella

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13
Q

SPECIAL STAINS

Leifson

A

Flagella

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14
Q

SPECIAL STAINS

Feulgen

A

DNA

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15
Q

SPECIAL STAINS

Levaditi’s

A

Spirochetes

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16
Q

HOT OR COLD

Kinyoun Method

A

Cold

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17
Q

HOT OR COLD

Ziehl-Neelsen

A

Hot

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18
Q

ZN or Kinyoun:

Which one is safer?

A

Kinyoun

Because ZN has open flames (toxic)

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19
Q

FLAGELLA

Other term for monotrichous

A

Polar

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20
Q

FLAGELLA

Flagella at both ends

A

Amphitrichous

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21
Q

FLAGELLA

Many tufts in one end

A

Lophotrichous

22
Q

FLAGELLA

Occur all around the bacterium

A

Peritrichous

23
Q

PILI OR FIMBRIAE

for DNA exchange

A

Pili

24
Q

T/F:

Capsules act as virulence factors

A

True

25
Q

T/F:

Bacteria are eukaryotes

A

False

prokaryotes

26
Q

G+ or G-

Which one has a periplasmic space?

A

G-

27
Q

G+ or G-

Which one has a thick murein layer?

A

G+

28
Q

thick cell wall made up of alternating chains of mycolic acid and trehalose sugars

A

Acid Fast Cell Wall

29
Q

Identify

G+ and G- cells that lose their cell walls

A

L-forms

30
Q

Identify:

Contains most/all of the genetic material

A

Mesosomes

31
Q

CAPSULE

Polypeptide D-Glutamic Acid Containing

what bacteria?

A

Bacillus anthracis

32
Q

CAPSULE

Hyaluronic Acid Containing

what bacteria?

A

Pasteurella multocida

33
Q

FLAGELLA

Motility is best observed at __ degC

A

25degC

34
Q

Composition of Endospores

A

dipicolinic acid calcium complex

35
Q

INCLUSIONS

contains sulfur containing granules

A

Thiobacillus

36
Q

INCLUSIONS

(2) have bipolar bodies

A
  • Yersinia pestis
  • Pasteurella multocida
37
Q

T/F:

Basic dyes are anionic

A

False

remember BC+ (basic/cationic +, bind to - charged)

38
Q

T/F:

Acid Dyes are anionic

A

True

remember BC+ and AN-

39
Q

Gram staining is an example of: simple or differential staining?

A

Differential

2 or more dyes

40
Q

CAPSULE

Klebsiella pneumoniae is

A

Polysaccharide Containing

41
Q

T/F:

Only bacteria produce biofilms

A

False

also fungi

42
Q

ACID OR BASIC

rose bengal

A

acid stain

43
Q

ACID OR BASIC

safranin

A

basic

44
Q

most popular negative staining procedure

A

India Ink

45
Q

__ staining is used to demonstrate the capsule

A

Indirect/Relief/Negative

46
Q

Enumerate

4 reagents in gram staining

A
  • Primary Stain
  • Mordant
  • Decolorizer
  • Secondary Stain
47
Q

4 reagents in gram staining

this is for stronger binding

A

mordant

48
Q

staining method for Nocardia and Mycobacterium tuberculosis

A

Acid Fast Staining

49
Q

Primary stain in ZN method

A

Carbolfuchsin

50
Q

ZN or KInyoun?

Mordant is Tergitol

A

Kinyoun