lec 12

Question 1

Site specific recomination

Answer 1

recbomibnation between smaller stretches of homology (limited stretch of similarity)
-regions with similar sequence will flank genes that can be moved

three functions:
Inversions:
Insertion:
Deletions:

general recombination: large sequences of dna recombination

Question 2

What components are needed for site directed recombination

Answer 2

1) Rearrangement enxymes: Recombinases
-cleave and rejoin dna using a covalent protein dna intermediate
a) site specific endonucleus acrivity (cleavage)
b) Dna ligase activity

2) recombination site (specific sequence)
-place where dna exchange will occur
a)recombinase recognises sequence
b) has to be non-palindromic sequences