Last set review Flashcards
1
Q
- Eukaryotic RNA polymerase I genes code for
a. mRNAs.
b. tRNAs.
c. small nuclear RNAs and small cytoplasmic RNAs.
d. ribosomal RNAs.
A
ribosomal RNAs.
2
Q
- Mitochondrial genes are transcribed by
a. RNA polymerase I.
b. RNA polymerase II.
c. RNA polymerase III.
d. a separate mitochondrial RNA polymerase.
A
a separate mitochondrial RNA polymerase.
3
Q
- The first step in the formation of a transcription complex for mRNA transcription is the binding of _______ to the TATA box.
a. TFIA
b. TFIIA
c. TFIIIA
d. TFIID
A
TFIID
4
Q
- The large multi-subunit complex that links the general transcription factors to the gene-specific transcription factors is called
a. the transcription complex.
b. Mediator.
c. the operator.
d. TBP.
A
the operator
5
Q
- Release of RNA polymerase II to initiate transcription appears to be the direct result of the
a. binding of TAFs to the polymerase.
b. unwinding of the DNA by helicases.
c. phosphorylation of RNA polymerase by a protein kinase.
d. removal of the nucleosome occupying the promoter site.
A
phosphorylation of RNA polymerase by a protein kinase.
6
Q
- A reporter gene is one that
a. causes other genes to be expressed.
b. can produce a product that is easily detected when expressed under the control of a regulatory sequence to which it has been ligated and cloned.
c. is always on.
d. is turned on by the products of other genes.
A
can produce a product that is easily detected when expressed under the control of a regulatory sequence to which it has been ligated and cloned
7
Q
- A major difference between eukaryotic and prokaryotic RNA polymerases is that eukaryotic polymerases
a. use a set of transcription factors to bind to and initiate transcription.
b. use sigma () factors to initiate transcription.
c. start from promoters.
d. start from origins of replication.
A
use a set of transcription factors to bind to and initiate transcription
8
Q
- Enhancers usually are _______ sequences that require _______ to regulate a gene.
a. cis-acting; other cis-acting sequences
b. cis-acting; trans-acting factors
c. trans-acting; trans-acting factors
d. Both a and b
A
Both a and b
9
Q
- Enhancers have been found in
a. eukaryotic cells.
b. prokaryotic cells.
c. yeast.
d. Both a and b
A
Both a and b
10
Q
- The immunoglobulin enhancer has binding sites for _______ regulatory protein(s).
a. one specific
b. multiple positive- and negative-acting
c. multiple positive-acting
d. multiple negative-acting
A
multiple positive- and negative-acting
11
Q
- DNA sequences that prevent enhancers from acting on promoters located in adjacent domains are called
a. insulators.
b. isolators.
c. repressors.
d. boundaries.
A
insulators