Last minute learn Flashcards
Name another 2 Src kinases other than Src Kinase
Lck, shares recognition motif Y*EEI on SH2 domain
ABLE - which share around 60% homology with Src: DFG motif (on kinase domain)- 3AAs next to phosphotyrosine on the activation loop - inactive, facing out of active site, active, goes into active site, stabilizing ATP molecule for catalysis
Reference for string model + residues of the switches
Wittinghofer 2011
Switch 1 = Thr35
Switch 2 = Gly60
Interaction of Ras and HIV-Nef with Ras - methods to study
Peptide binding assay by deletion and immobalisation, identifies interacting peptide sequences
Recognition motif for Nef/ general consensus sequence for Raf stimulation
X(D/E)DPXXEX
S phase Cyclin A/cdk2 also does what
Phosphorylates cohesins - facilitate formation of sister chromatids
Fibrillin/vIL6 diseases
Fibrillin mutations = marfan syndrome
vIL6 derived from Kaposi’s sarcoma associated herpes virus
Once integrins cluster:
tyrosine phosphorylation
Actin rearrangment
potential bacterial uptake
Residues of Src kinase
Dephosphorylation of Y527
Phosphorylationof Y416 on the activation loop
3 loops of SH3v
n-Src-loop and RF loop, both on the ligand binding site, modulate/assist binding
distal loop - interacts with other parts if the protein to stablaise it/binding
CHR recognition domain structure
EB(startA),G(end)FCC’
GH-GH sites
site 1 - Helix D + AB loop (large)
GHR: W104
GH:K172, T175 (helix D)
Site 2 - Helix A +C (small)/weaker interaction
GHR: W104
GH: G120, D116 (helix C)
GCSF-GCSF sites
Site 1 - not used
Site 2 - Y173
Site 3 - I88, D90, Q91
IL6/IL6a/gp130 sites
Site 1: IL6 (A/D) + IL6-Ra (D2/D3)
IL6: K171, E175, R179 (Helix D), IL6-Ra: W229 hotspot
Site 2(a): IL6 (A/C) F169+ gp130 (D2/D3)B
Site 3(a): IL6 (D + ABloop) Trimer 2, gp130 (D1) Trimer 1
3 Areas of cytokines that can be engineered
Receptor affinity
Receptor geometry
Receptor composition
G elements required for what?
Nucleotide recognition
G1 = phosphate vinding loop
