Laser Scanning Confocal Microscope Flashcards
1
Q
What is added to the cell / specimen / organelle?
A
a flourescent dye
2
Q
What is focused on part of the cell?
A
laser beam
3
Q
What flouresces from objects and how?
A
light at different depths within the cells
4
Q
What sits in front of the detector?
A
a pinhole
5
Q
What does the pinhole do?
A
it eliminates the out of focused light from different parts of the cells
6
Q
What are the advantages of laser scanning confocal microscopes?
A
higher resolution than light microscopes
can view objects inside cell
7
Q
What are the disadvantages of a cell?
A
more expensive than light microscopes
lower resolutions than T.E.M microscopes
