Labsafety

Question 1

intended to protect hospital personnel from bloodborne infections

Answer 1

Universal precautions

Question 2

True or False: Blood and all body fluids, including secretions and excretions except sweat, regardless of whether visible blood is present, are considered infectious.

Answer 2

True

Question 3

Must be done after touching blood, body fluids, secretions, excretions, and any items considered contaminated.

Answer 3

Handwashing

Question 4

Should be worn when handling blood, body fluids, secretions, excretions, and contaminated items.

Answer 4

Gloves

Question 5

must be worn for potential splashes or sprays of blood, body fluids, secretions and excretions

Answer 5

Mask, eye protection, or face shield

Question 6

Must be worn to protect skin and clothing when contact with blood, body fluids, secretions and excretions could occur.

Answer 6

Laboratory coats

Question 7

Must be implemented with care to prevent injuries with sharps, needles, and scalpels. Placed in appropriate puncture-resistant containers after use.

Answer 7

Appropriate sharps disposal

Question 8

Must include procedures for routine care, cleaning and disinfection of environmental surfaces.

Answer 8

Environmental control

Question 9

added precautions that are used when patient is known or suspected to be infected or colonized with an infectious agent. requires extra measures to prevent spread

Answer 9

Transmission-based precautions

Question 10

What are the categories of transmission-based precautions?

Answer 10

Contact
Droplet
Airborne

Question 11

used to stop the spread of infectious agents transmitted through direct/indirect contact with patient or environment

Answer 11

Contact precautions

Question 12

used to stop the spread of infectious agents that can be transmitted by close respiratory contact or by exposure of mucous membranes to respiraroty secretions.

Answer 12

Droplet precautions

Question 13

precautions used for M.tuberculosis, varicella virus, and rubeola virus, that can remain infectious over long distances

Answer 13

Airborne precautions

Question 14

What is the sequence for putting on PPE?

Answer 14

Gown, mask/respirator, goggles/face shield. gloves

Question 15

sequence for removing PPE?

Answer 15

gloves, goggles/face shield, gown, mask/respirator, wash hands or use an alcohol-based hand sanitizer immediately after removing all ppe

Question 16

designed by Charles Baldwin in 1966, symbol is used in labeling materials that could pose a health risk.

Answer 16

Biohazard symbol

Question 17

What is the triad in the chain of infectiom

Answer 17

Agent, Host, Environment

Question 18

When a microorganism invades the body, multiplies, and causes injury or disease: communicable and nosocomial HAIs

Answer 18

Infection control

Question 19

pathogenic microbe such as virus, bacteria, fungus, protozoa, rickettsia

Answer 19

Infectious (causative) agent

Question 20

source of infection, agent, or place where the microbe could grow, survive, and multiply which could be in humans, animals, food, water, soil or equipment

Answer 20

Reservoir

Question 21

A way or manner where and infectious agent can leave the reservoir host which could be through secretion and exudates, tissue specimens, blood, feces or urine

Answer 21

Exit pathway

Question 22

Airborne, direct, indirect, vector, and vehicle

Answer 22

Means of transmission

Question 23

refers to the way an infectious agent enters a host susceptible to infection includes body orifices, mucous membranes, and breaks in skin

Answer 23

Entry pathway

Question 24

refers to the way an infectious agent enters a host susceptible to infection includes body orifices, mucous membranes, and breaks in skin

Answer 24

Entry pathway

Question 25

someone prone to infection due to age, health or immune status.

Answer 25

susceptible host

Question 26

What are the steps to clean blood spill?

Answer 26

1. Wear PPE 2. use forceps for discarding the broken glass on a sharps container 3. Cover with absorbent paper towels 4. Flood area with 10% bleach solution 5. Let sit for 10 min 6, Clean up area with paper towels 7. Dispose in biohazard bad 8. Repeat if necessary

Question 27

what are the 2 major sources of biohazard in microbiology laboroatory?

Answer 27

1. Processing of patient specimens 2. Handling actively growing cultures of microorganisms

Question 28

What are the major routes of LAIs in Clinical laboratory?

Answer 28

1. Parenteral inoculations 2. Spills and splashes onto skin or mucous membranes 3. Ingestions 4. Inhalation of infectious aerosols

Question 29

Risk group of a microorganism that is unlikely to cause human or animal disease.

Answer 29

RG1

Question 30

A pathogen that can cause human or animal disease but unlikely serious hazard. May cause serious infection but effective treatment is available.

Answer 30

RG2

Question 31

It has no or low individual and community risk.

Answer 31

RG1

Question 32

It has moderate individual risk and low community risk.

Answer 32

RG2

Question 33

Risk group of a pathogen that usually causes serious human or animal disease but does not ordinarily spread from one to another. Effective treatment available.

Answer 33

RG3

Question 34

It has high individual risk and low community risk.

Answer 34

RG3

Question 35

Risk group of a pathogen causes serious human, readily transmitted from one to another. Treatment not available.

Answer 35

RG4

Question 36

It has high individual and community risk.

Answer 36

RG 4

Question 37

Dangerous and exotic, posing a high risk of aerosol-transmitted infection, frequently FATAL and without treatment.

Answer 37

BSL-4

Question 38

Ebola virus and smallpox virus are under BSL?

Answer 38

BSL-4

Question 39

Either indigenous or exotic, can cause serious or potentially lethal disease through respiratory transmission.

Answer 39

BSL-3

Question 40

Example of this BSL are: Yersinia pestis (plague), M.tuberculosis, SARS, rabies virus, West Nille virus, hantaviruses

Answer 40

BSL-3

Question 41

BSL of a moderate potential hazard to personnel and environment. Cause mild diseases to humans, or are difficult to contract (transmit) via aerosol

Answer 41

BSL-2

Question 42

BSL exmaples: Hepatitis A, Streptococcus pyogenes, Borrella burgdorferi (Lyme disease), Salmonella species

Answer 42

BSL-2

Question 43

Not known to consistently cause disease in adult, and minimal to lab personnel

Answer 43

BSL-1

Question 44

BSL example: Saccharomyces cerevisiae, E.coli, K-12 and non-infectious bacteria

Answer 44

BSL-1

Question 45

Laboratory work that can be conducted on open benchtops

Answer 45

BSL1

Question 46

Laboratorians in this Biosafety level should receive immunizations or tests for agents handled with potential

Answer 46

BSL2

Question 47

A Biosafety manual must be developed and updated, recommend BSC class 2. Use of needles are restricted

Answer 47

BSL 2

Question 48

Potential for aerosol transmission, personnel should have specific training

Answer 48

BSL 3

Question 49

Lab should be separated from other parts of the building and be accessed through two self-closing doors, Anteroom may be used.

Answer 49

BSL 3

Question 50

Requires a ducted air ventilation system that must provide directional airflow. Pulls air from clean areas toward potentially contaminated areas

Answer 50

BSL3

Question 51

ceiling and floor must be solid and seams must be sealed. all parts must be constructed for easy cleaning and decontamination

Answer 51

BSL 3

Question 52

agents that are dangerous and exotic are under this bsl, facility is isolated or in a separate building.

Answer 52

BSL 4

Question 53

In this laboratory, all work is performed within a class III BSC

Answer 53

Cabinet Laboratory

Question 54

In this lab, personnel wear a positive pressure protective suit to perform all work

Answer 54

Suit Laboratory

Question 55

The BSL 4 lab has a dedicated type of ventilation system that is filtered through a HEPA filter before exhausted. What is this ventilation system?

Answer 55

Nonrecirculating ventilation system