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Step 1 Biochemistry-First Aid 2017 > Laboratory techniques > Flashcards

Laboratory techniques Flashcards

1
Q

Laboratory procedure used to amplify a desired fragment of DNA

A

PCR

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2
Q

Blotting procedure used to analyze DNA

A

Southern blot

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3
Q

Blotting procedure that identifies DNA-binding proteins like transcription factors

A

Southwestern blot

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4
Q

Blotting procedure used to analyze RNA

A

Northern blot

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5
Q

Confirmatory test for HIV after positive ELISA is what type of blotting procedure

A

Western blot

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6
Q

Blotting procedure useful for studying mRNA levels

A

Northern blot

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7
Q

Blotting procedure used to analyze proteins

A

Western blot

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8
Q

Lab technique used to assess size, granularity, and protein expression of individual cells in a sample

A

Flow cytometry

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9
Q

Used to profile gene expression levels of multiple genes simultaneously to study diseases and treatments

A

Microarrays

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10
Q

Commonly used in workup of hematologic abnormalities

A

Flow cytometry

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11
Q

Lab technique use to detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs)

A

Microarrays

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12
Q

Immunologic test used to detect the presence of either a specific antigen or antibody in a patient’s blood sample

A

Enzyme-linked immunosorbent assay

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13
Q

Enzyme-linked immunosorbent assay detection involves the use of what?

A

Antibody linked to an enzyme

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14
Q

What produces a detectable signal in Enzyme-linked immunosorbent assay

A

Added substrate reacting with enzyme

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15
Q

Lab technique used to diagnose chromosomal imbalances

A

Karyotyping

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16
Q

Lab technique used for specific localization of genes and direct visualization of chromosomal anomalies at the molecular level

A

Fluorescence in situ hybridization

17
Q

Chromosome abnormality with fluorescence signal that corresponds to one chromosome is found in a different chromosome

A

Translocation

18
Q

Chromosome abnormality with extra site of fluorescence on one chromosome relative to its homologous chromosome

A

Duplication

19
Q

Chromosome abnormality with no fluorescence on a chromosome compared to fluorescence at the same locus on the second copy of that chromosome

A

Microdeletion

20
Q

Process causing a recombinant DNA molecule to self-perpetuate

A

Cloning

21
Q

Unique property of cDNA

A

Lacks introns

22
Q

Function of RNA interference

A

Promote degradation of target mRNA

23
Q

Cre-lox system

A

Manipulates genes at specific developmental points

Step 1 Biochemistry-First Aid 2017 (5 decks)

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