Laboratory techniques Flashcards
Laboratory procedure used to amplify a desired fragment of DNA
PCR
Blotting procedure used to analyze DNA
Southern blot
Blotting procedure that identifies DNA-binding proteins like transcription factors
Southwestern blot
Blotting procedure used to analyze RNA
Northern blot
Confirmatory test for HIV after positive ELISA is what type of blotting procedure
Western blot
Blotting procedure useful for studying mRNA levels
Northern blot
Blotting procedure used to analyze proteins
Western blot
Lab technique used to assess size, granularity, and protein expression of individual cells in a sample
Flow cytometry
Used to profile gene expression levels of multiple genes simultaneously to study diseases and treatments
Microarrays
Commonly used in workup of hematologic abnormalities
Flow cytometry
Lab technique use to detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs)
Microarrays
Immunologic test used to detect the presence of either a specific antigen or antibody in a patient’s blood sample
Enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay detection involves the use of what?
Antibody linked to an enzyme
What produces a detectable signal in Enzyme-linked immunosorbent assay
Added substrate reacting with enzyme
Lab technique used to diagnose chromosomal imbalances
Karyotyping