Laboratory Safety Rules and Regulations Flashcards
Public Law 91-596 is also known as the ____.
Occupational Safety and Health Act
Occupational Safety and Health Act is also known as ____.
Public Law 91-596
The goal of Public Law 91-596 is to ____.
provide all employees with a safe work environment
____ is authorized to conduct on-site inspection to determine whether an employer is complying with the mandatory standards.
Occupational Safety and Health Administration (OSHA)
Identify if Employer’s or Employee’s Responsibility
Establish laboratory work methods and safety policies
Employer’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Provide supervision and guidance
Employer’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Provide safety information, training, PPE, and medical surveillance
Employer’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Provide and maintain equipment and laboratory facilities that are free of recognized hazards and adequate for the tasks required
Employer’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Know and comply with the established safe work practices
Employee’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Have a positive attitude toward supervisors, co-workers, facilities, and safety training
Employee’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Be alert and give prompt notification of unsafe conditions or practices
Employee’s Responsibility
Identify if Employer’s or Employee’s Responsibility
Engage in the conduct of safe work practices and use of PPE
Employee’s Responsibility
The ____ developed a standard hazard identification system, which has been adopted by many clinical laboratories.
National Fire Protection Association (NFPA)
Hazards under the NFPA symbol
- Red: Flammability
- Yellow: Reactivity/Instability
- White: Special
- Blue: Health
A proper label should consist of ____.
- Statement of Hazard
- Hazard Class
- Safety Precautions
- NFPA Hazard
- Fire extinguisher type
- Safety instructions
- Formula weight
- Lot number
All laboratories are required to have ____.
- Safety showers
- Eyewash stations
- Fire extinguisher
- Periodical testing and inspection
Safety showers must have ____.
30 - 50 gallons of water at 20 - 50 lbs psi
Eyewash stations must be accessible within ____.
100 ft or 10 s travel
____ must be used for manipulating all types of liquids in the laboratory.
Mechanical pipetting devices
____ are required to contain and expel noxious and hazardous fumes from chemical reagents.
Fume Hoods
Fume hoods should be visually inspected for ____.
Blockage
____ remove particles that may be harmful to the employee who is working with potentially infectious biologic specimens.
Biosafety Cabinets
____ is the measured air speed at an inlet or outlet of a heating ventilation and air conditioning system (HVAC).
Face Velocity
HVAC stands for ____.
Heating Ventilation and Air Conditioning System
____ are exhaust systems of BSC that traps all infectious agent and ensure that only the microbe-free exhaust air is discharged.
HEPA Filter
HEPA stands for ____.
High Efficiency Particulate Air
Classification of Infective Microorganisms by Risk Group
- Risk Group 1 - No or Low Individual and Community Risk
- Risk Group 2 - Moderate Individual Risk, Low Community Risk
- Risk Group 3 - High Indiviual Risk, Low Community Risk
- Risk Group 4 - High Individual and Community Risk
Classification of Infective Microorganisms by Risk Group
A microorganism that is unlikely to cause human or animal disease
Risk Group 1
Classification of Infective Microorganisms by Risk Group
A pathogen that can cause human or animal disease but is unlikely to be a serious hazard to community.
Risk Group 2
Classification of Infective Microorganisms by Risk Group
A pathogen that usually causes serious human or animal disease but does not ordinarily spread.
Risk Group 3
Classification of Infective Microorganisms by Risk Group
A pathogen that usually causes serious disease and that can be readily transmitted from one to another.
Risk Group 4
____ are required for the storage of flammable liquids.
Steel safety cabinets with self-closing door
____ may be used to store flammable materials.
Explosion-proof refrigerators
Larger cylinders should be transported with valve caps on, using ____.
handcarts
A full body PPE is called ____.
Hazmat suit
Donning Sequence of PPE
- Gown
- Mask
- Goggles or Face Shield
- Gloves
Doffing Sequence of PPE
- Gloves
- Goggles or Face Shield
- Gown
- Mask
All blood samples and other body fluids should be processed using the ____.
universal precautions
N-95 protection
95% of particles >0.3 um
Known or suspected infectious specimens are labeled ____ if the pathogen can be readily transmitted to humans or animals.
Infectious substances
____ are those tested as routine screening or for initial diagnosis.
Diagnostic specimens
Flammable or Combustible materials are classified according to ____.
Flash point
____ is the temperature at which sufficient vapor is given off to form an ignitable mixture with air.
Flash point
Flash point of flammable materials
below 37.8 C (<100 F)
Flash point of combustible materials
above 37.8 C (>100 F)
Commonly used flammable and combustible solvents
- Acetone
- Benzene
- Ethanol
- Heptane
- Isopropanol
- Methanol
- Toluene
- Xylene
An example of a flammable or combustible gas
Hydrogen
An example of a flammable or combustible solid
Paraffin
Examples of Corrosive Chemicals
- Acids
- Bases
____ is liberated if alkali metals are mixed with water or acids, which can cause combustion.
Hydrogen
The mixture of ____ and ____ generates heat and may be explosive.
oxidizing agents and reducing agents
____ is a common example of a known carcinogen.
Benzidine
____ is a chemical reaction that involves the rapid oxidation of a combustible material or fuel with a subsequent variation of heat and light.
Fire
Classification of Fires
- Class A: Combustible solids
- Class B: Flammable liquids/gasses
- Class C: Electrical equipments
- Class D: Combustible/reactive metals
PASS stands for ____.
- Pull pin
- Aim nozzle
- Squeeze trigger
- Sweep nozzle
RACE stands for ____.
- Rescue
- Alarm
- Contain
- Extinguish/Escape
Store tanks in a ____ position.
vertical
____ is the most widely used cryogenic fluid in the laboratory.
Liquid Nitrogen
____ should be added to help eliminate bumping/boilover when liquids are heated.
Glass beads or boiling chips
