Laboratory Safety and Regulations Flashcards
OSHA means _____
Occupational Safety and Health
Administration
CLSI means _____
Clinical and Laboratory Standards
Institute
CDC means _____
Centers for Disease Control and
Prevention
CAP means _____
College of American Pathologists
TJC means _____
The Joint Commission
The Joint Commission is formerly _____
JCAHO
An agency within the U.S. Department of Labor to set levels of safety and health for all workers in the United States
OSHA
A nonprofit organization that sets voluntary consensus standards for all areas of clinical laboratories
CLSI
A Federal agency that carries out mandated public health laws and reporting requirements
CDC
An agency that provides accreditation and proficiency testing for laboratories
CAP
An independent, not-for-profit organization that accredits and certifies health-care organizations and programs in the United States
TJC
What is/are the source for the chemical type of laboratory hazard?
Preservatives and reagents
What is/are the possible injury for the chemical type laboratory hazard?
Exposure to toxic carcinogen
Caustic agents
What is/are the source for the sharps type of laboratory hazard?
Needles, lancets, and broken glass
What is/are the possible injury for the sharps type of laboratory hazard?
Cuts
Punctures
Blood-borne pathogen exposure
What is/are the source for the electrical type of laboratory hazard?
Ungrounded or wet equipment
Frayed cords
What is/are the possible injury for the electrical type of laboratory hazard?
Burns or shock
What is/are the source for the fire/explosive type of laboratory hazard?
Bunsen burners
Organic chemicals
What is/are the possible injury for the fire/explosive type of laboratory hazard?
Burns
Dismemberment
What is/are the source for the physical type of laboratory hazard?
Wet floors
Heavy boxes
Patients
What is/are the possible injury for the physical type of laboratory hazard?
Falls
Sprains or strains
What is/are the source for the radiation type of laboratory hazard?
Equipment and radioisotopes
What is/are the possible injury for the radiation type of laboratory hazard?
Radiation exposure
What is/are the source for the biological type of laboratory hazard?
Infectious agent
What is/are the possible injury for the biological type of laboratory hazard?
(Infections) - Bacterial, Fungal, Viral, or Parasitic
Identify the Risk Factor:
Includes pathogens on the hands of medical personnels, invasive procedures (intubation, indwelling vascular lines, urine catheterization)
Iatrogenic Risk Factors
Identify the Risk Factor:
Contaminated airconditioning systems, contaminated water systems, staffing and physical layout of the facility
Organizational Risk Factor
Identify the Risk Factor:
Severity of illness, underlying state of the patient and length of stay
Patient Risk Factor
List down the Potential Hazards:
- Electric shock, toxic vapors, compressed gases, flammable liquids, radioactive material, and corrosive substances
- Mechanical trauma
- Poisons
- Inherent risks of handling biologic materials
List down the Primary Cause of Accidents:
- Unsafe Acts (not always recognized by personnel)
- Unsafe Environmental Conditions
First rule of self protection is _____
Alertness at all times
Safety begins with the recognition of hazards
and is achieved through:
- Application of common sense
- Safety-focused attitude
- Good personal behavior
- Good housekeeping in all laboratory work and storage areas
- Continual practice of good laboratory technique
Preventive Measures:
- Annual safety reviews
- Safety drills
- General consciousness
- Appropriate orientation to safety rules
- Safe work environment
Heat sterilization time and temperature
250 degree Celsius for 15 minutes
Preferred amount and time of Ethylene Oxide for inactivation:
450/500 mg/L at 55 to 60 degree Celsius
Percentage of Glutaraldehyde for inactivation
2% Glutaraldehyde
Percentage of Hydrogen peroxide for inactivation
10% Hydrogen peroxide
Safety showers must deliver how many gallons of water per minutes?
30-50 gallons
Safety showers deliver 30 - 50 gallons of
water per minute at _____
20-50 pounds per square inch (psi)
Expel noxious and hazardous fumes from chemical reagents
Fume Hoods
How do we check the airflow direction?
By placing a tissue paper at the opening of the hood
This is done to locate no flow or turbulent areas in the working space
Smoke Testing
Remove particles that may be harmful to the employee who is working with potentially
infectious biologic specimens
Biosafety Cabinet
All Clinical Laboratory should have:
Chemical Hygiene Plan
Exposure Control Plan
Copy of MSDS
Disinfect the spill site using approved disinfectant or _____ using appropriate contact time
10% bleach
Identify the Category of Exposure:
Daily exposure to blood and body fluids
Category I
Identify the Category of Exposure:
Regular exposure to blood and body fluids
Category II
Identify the Category of Exposure:
No exposure to blood and body fluids
Category III
How many Categories of Exposure is there?
Three Categories of Exposure
In the NFPA Labeling System, what color designates flammability?
Red
Red color in the NFPA Labeling System indicates _____
Flammabiliy
In the NFPA, what color indicates Health Hazard?
Blue
Blue color in the NFPA Labeling System indicates _____
Health Hazard
In the NFPA, what color indicates Reactivity Level?
Yellow
Yellow color in the NFPA Labeling System indicates _____
Reactivity Level
In the NFPA, what color indicates Special Considerations/Personal Protection?
White
White color in the NFPA Labeling System indicates _____
Special Considerations/Personal Protection
Communicates chemical hazard
information through the use of
colors, numbers and letters of the
alphabet
Hazardous Materials Identification
System (HMIS)
Identify if HMIS or NFPA:
Intended to be used by employers and
workers
HMIS
Identify if HMIS or NFPA:
Intended to be used by emergency response personnel
NFPA
Identify if HMIS or NFPA:
Bar shape
HMIS
Identify if HMIS or NFPA:
Diamond shape
NFPA
Identify if HMIS or NFPA:
Covers both acute and chronic health
hazards
HMIS
Identify if HMIS or NFPA:
Only covers acute health hazards
NFPA
Identify if HMIS or NFPA:
Shows recommended PPE
HMIS
Identify if HMIS or NFPA:
Used to display other special hazards
NFPA
SDS Section 1
Identification
SDS Section 2
Hazard Identification
SDS Section 3
Ingredient Information
SDS Section 4
First Aid Procedures
SDS Section 5
Fire-fighting Procedures
SDS Section 6
Accidental-release Measures
SDS Section 7
Handling and Storage
SDS Section 9
Exposure Controls and Personal Protection
SDS Section 10
Stability and Reactivity
SDS Section 11
Toxicological Information
SDS Section 12
Ecological Information
SDS Section 13
Disposal Consideration
SDS Section 14
Transport Information
SDS Section 15
Regulatory Information
SDS Section 16
Other Information, Including Date of Preparation or Last Revision
Identify the Section on SDS:
Identification
Section 1
Identify the Section on SDS:
Hazard Identification
Section 2
Identify the Section on SDS:
Ingredients Information
Section 3
Identify the Section on SDS:
First Aid Procedures
Section 4
Identify the Section on SDS:
Fire-fighting Procedures
Section 5
Identify the Section on SDS:
Accidental-release Measures
Section 6
Identify the Section on SDS:
Handling and Storage
Section 7
Identify the Section on SDS:
Exposure Controls and Personal Protection
Section 8
Identify the Section on SDS:
Physical and Chemical Properties
Section 9
Identify the Section on SDS:
Stability and Reactivity
Section 10
Identify the Section on SDS:
Toxicological Information
Section 11
Identify the Section on SDS:
Ecological Information
Section 12
Identify the Section on SDS:
Disposal Considerations
Section 13
Identify the Section on SDS:
Transport Information
Section 14
Identify the Section on SDS:
Regulatory Information
Section 15
Identify the Section on SDS:
Other Information, Including Date of Preparation or Last Revision
Section 16
The temperature at which sufficient vapor is given off to form an ignitable mixture with air
Flash Point
Injurious to the skin or eyes by direct contact or to the tissue of the respiratory and gastrointestinal tracts if inhaled or ingested
Corrosive Chemicals
Spontaneously explode or ignite or that evolve heat or flammable or explosive gases
Reactive Chemicals
Radiation monitoring utilizes film badge or survey meter. The maximum permissible dose is _____
5000 mrem/year whole body
A test that involves wiping laboratory surfaces with moistened absorbent material and the radiation contained in each wipe is counted
Wipe Test/Leak Test
Compressed Gases Hazards
Danger of Fire
Explosion
Asphyxiation
Mechanical Injuries
Identify the Class of Fire:
Ordinary combustible: Wood, Paper, Cloth, etc
Class A
Identify the Class of Fire:
Flammable Liquids/Gasses and Combustible Petroleum Products
Class B
Identify the Class of Fire:
Energized Electrical Equipment, Motors, Switches
Class C
Identify the Class of Fire:
Combustible/Reactive Metals (Mg, Na, K)
Class D
Identify the Class of Fire:
Detonation
Class E
What type of Fire Extinguisher is used for Class A type of Fire?
ABC Dry Chemical
Halotron
Water
Foam
What type of Fire Extinguisher is used for Class B type of Fire?
ABC Dry Chemical
BC Dry Chemical
Purple K
Carbon Dioxide
Halotron
Foam
What type of Fire Extinguisher is used for Class C type of Fire?
ABC Dry Chemical
BC Dry Chemical
Purple K
Carbon Dioxide
Halotron
What type of Fire Extinguisher is used for Class D type of Fire?
Dry Powder
What type of Fire Extinguisher is used for Class K type of Fire?
Wet Chemical
_____ requires that all health-care institutions post evacuation routes and detailed plans to follow in the event of a fire
The Joint Commission on Accreditation of Healthcare Organizations (JCAHO)
The “R” in the RACE acronym means _____
Rescue
The “A” in the RACE acronym means _____
Activate
The “C” in the RACE acronym means _____
Contain
The “E” in the RACE acronym means _____
Extinguish
How to operate the Fire Extingusiher?
PULL pin
AIM noozle
SQUEEZE trigger
SWEEP noozle
Most widely used cryogenic fluids (liquefied gases) in the laboratory
Liquid Nitrogen
Help eliminate bumping/boil over when liquids are heated
Glass Beads
Enumerate the Hierarchy of Controls from the Most Effective to the Least Effective
Elimination
Substitution
Engineering Controls
Administration Controls
Personal Protective Equipment
Completely remove the hazard from the workplace or the process, thus eliminating the risk entirely
Elimination
Replace the hazardous material, process, or equipment with a safer alternative.
Substitution
Implement physical modifications to the workplace or equipment to isolate workers from the hazard or reduce
exposure.
Engineering Controls
Develop and implement safety policies, procedures, and training to reduce the likelihood of exposure to hazards.
Administrative Controls
Provide workers with appropriate PPE, such as gloves, respirators, or protective clothing, to protect against the identified hazard.
Personal Protective Equipment (PPE)
What is the 4 Basic Waste Disposal Technique?
Flushing down the drain
Incineration
Landfill burial
Recycling
Special waste from health care facilities
Medical Waste